TY - JOUR
T1 - Expression of Vimentin and GFAP protein of cerebral cortex and its impact on corticogenesis disorder as a result of 2-methoxyethanol
AU - Irnidayanti, Yulia
AU - Darmanto, Win
PY - 2013
Y1 - 2013
N2 - One of the plastic base material, widely used in the plastics industry in various countries, is a ester phthalate. These compounds will be oxidized in the body to 2-methoxyethanol (2-ME). Effect of 2-ME on human health and the environment depends on the number, duration and frequency of exposure. 2-ME and its metabolites in the body can damage cells and tissues. The body can be exposed by 2-ME through the air, water and soil. Western blot results showed that the protein Vimentin was detectable in the control group at GD-11 to 17, meanwhile GFAP protein was detachable in the control group at GD- 12 to GD-18. After administration 2-ME, the expression of Vimentin protein were changed, and started at GD- 12 up to GD-18. whereas the expression of GFAP protein began at GD- 11 up to GD-17. The Changes on timetable protein expression of Vimentin and GFAP affect corticogenesis disorder. The disorder caused by the existence of these proteins as a result of 2-Methoxyethanol. Disorder of corticogenesis process were sub-plate and cortical plate of the cerebral cortex of fetus brains of mice at GD-18. Generally, it can be concluded that changes in protein expression of Vimentin and GFAP caused by 2-ME. The Vimentin more important during the period of fetal brain development. GFAP and Vimentin is a protein involved in response to damage caused by a teratogenic agent, so that cells in the cerebral cortex, has dedifferentiation.
AB - One of the plastic base material, widely used in the plastics industry in various countries, is a ester phthalate. These compounds will be oxidized in the body to 2-methoxyethanol (2-ME). Effect of 2-ME on human health and the environment depends on the number, duration and frequency of exposure. 2-ME and its metabolites in the body can damage cells and tissues. The body can be exposed by 2-ME through the air, water and soil. Western blot results showed that the protein Vimentin was detectable in the control group at GD-11 to 17, meanwhile GFAP protein was detachable in the control group at GD- 12 to GD-18. After administration 2-ME, the expression of Vimentin protein were changed, and started at GD- 12 up to GD-18. whereas the expression of GFAP protein began at GD- 11 up to GD-17. The Changes on timetable protein expression of Vimentin and GFAP affect corticogenesis disorder. The disorder caused by the existence of these proteins as a result of 2-Methoxyethanol. Disorder of corticogenesis process were sub-plate and cortical plate of the cerebral cortex of fetus brains of mice at GD-18. Generally, it can be concluded that changes in protein expression of Vimentin and GFAP caused by 2-ME. The Vimentin more important during the period of fetal brain development. GFAP and Vimentin is a protein involved in response to damage caused by a teratogenic agent, so that cells in the cerebral cortex, has dedifferentiation.
KW - 2-methoxyethanol
KW - Brain
KW - Cerebral cortex
KW - GFAP
KW - Vimentin
UR - http://www.scopus.com/inward/record.url?scp=84888402765&partnerID=8YFLogxK
U2 - 10.4067/S0717-95022013000300003
DO - 10.4067/S0717-95022013000300003
M3 - Article
AN - SCOPUS:84888402765
SN - 0717-9367
VL - 31
SP - 802
EP - 808
JO - International Journal of Morphology
JF - International Journal of Morphology
IS - 3
ER -