Evaluation of the culture of Spirulina sp. with Walne nutrient plus vitamin B12, KCl, NPK, ZA CaO and urea

Spirulina sp. is a blue green microalga (Cyanophyceae) with cells that colonize to form spiral-shaped filaments. Spirulina has active ingredients that can function as antioxidants. Spirulina can be processed into products that have economic value and various benefits. The aim of this study was to investigate the evaluation of the culture of Spirulina sp. both on the laboratory scale and semi-mass scale, using Walne nutrient plus vitamin B12, KCl, NPK, ZA, CaO and Urea. The working method started from the preparation of tools and materials, culture media, stock of fertilizers, sterilization of tools, seed maintenance, measurements of water quality parameter and growth calculations. Culture on a laboratory scale was done with a volume of 2 L of water, while culture on a semi-mass scale was done with a volume of 80 L of water. Culture of Spirulina sp. While reached its peak of population on day 9, accounting for 9.065 cells/mL, but then the figure decreased on the following days, while on a semi-mass scale the growth continued to show an increase until day 15 with a density of 23.275 cells/mL and had not experienced any signs of decline in population.