Abstract
The enhancement of bacterial resistance due to the use of antibiotics has become a major concern in aquaculture industry. Eco-friendly products are crucially needed to replace antibiotics in sustainable disease management. This research aims to determine the antibacterial activity of coffee leaves, extracted by different solvents, against Edwardsiella tarda and Streptococcus agalactiae. Both bacteria in this study have been known as pathogenic in fish culture. Through this research, an enhancement of coffee leaves waste is expected, in order to be used as natural antibiotics for infected fish treatment. Coffee leaves extract was obtained by maceration whilst the antibacterial activity was performed by disk diffusion and tube dilution methods. Ethyl acetate extract of coffee leaves has the highest antibacterial activity on both bacteria. Meanwhile, n-hexane extract has no antibacterial activity. The minimum inhibitory concentration (MIC) of ethyl acetate fraction is 6.25 mg mL-1 and the minimum bactericidal concentration (MBC) is 12.5 mg mL-1. In conclusion, ethyl acetate fraction of coffee leaves could be used as future sources of antibacterial agent for infected fish treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 2371-2377 |
| Number of pages | 7 |
| Journal | AACL Bioflux |
| Volume | 12 |
| Issue number | 6 |
| Publication status | Published - 30 Dec 2019 |
Keywords
- Antibiotics
- Bacteria
- Coffee leaves
- Extract
- Fish pathogen