TY - JOUR
T1 - Evaluation an Aqueous Extract of Irradiated and Non Irradiated of Orthosiphon Aristatus on Zebrafish Embryo (Danio Rerio) through Acute Toxicity Assay
AU - Balan, Santhra Segaran
AU - Manokaram, Punithaamalar
AU - Maya, Sitell
AU - Bahari, Hasnah
AU - Mohd Uzid, Mahathir
AU - Danial, Muhammad
AU - Abidin, Azrina Zainal
AU - Jesus, Arapoc Daryl
AU - Rahmadi, Mahardian
N1 - Publisher Copyright:
© 2022, Walailak University. All rights reserved.
PY - 2022/7/15
Y1 - 2022/7/15
N2 - Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irradiated and irradiated Orthosiphon aristatus at different dosage of 3, 6, 9 and 12 kilogray (kGy) on zebrafish embryo. Method: Survival rate, hatching rate, heart beat rate and scoliosis were observed and date were analysed using SPSS 25.00 windows. Result: The lethal dose (LC50) of non-irradiated Orthosiphon aristatus is 381.81 μg/mL compare to irradiated extract at 3 % is (371.27 μg/mL), 6 % (311.03 μg/mL), 9 % (160.72 μg/mL) and 12 % (140.18 μg/mL). Hatchability of zebrafish embryo for Orthosiphon aristatus extract decrease in the higher dosage of irradiated sample compared to non-irradiated sample. No presence of scoliosis was observed in all dosage of irradiated and non-irradiated of Orthosiphon aristatus. The heartbeat of zebrafish embryo treated with irradiated Orthosiphon aristatus specifically 12 % show decrease at 250 μg/mL concentration. Remaining dosage of irradiated and non-irradiated Orthosiphon aristatus show average heartbeat around 120-160 bpm. Conclusion: As conclusion, irradiated and non-irradiated of Orthosiphon aristatus is safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo.
AB - Background: Orthosiphon aristatus has been usually related to be powerful in curing many illnesses inclusive of post-partum remedy, anti-influence, rheumatism and stopping osteoporosis as a result of menopause. Objectives: Thus, this study was designed to differentiate the toxicity effect of non-irradiated and irradiated Orthosiphon aristatus at different dosage of 3, 6, 9 and 12 kilogray (kGy) on zebrafish embryo. Method: Survival rate, hatching rate, heart beat rate and scoliosis were observed and date were analysed using SPSS 25.00 windows. Result: The lethal dose (LC50) of non-irradiated Orthosiphon aristatus is 381.81 μg/mL compare to irradiated extract at 3 % is (371.27 μg/mL), 6 % (311.03 μg/mL), 9 % (160.72 μg/mL) and 12 % (140.18 μg/mL). Hatchability of zebrafish embryo for Orthosiphon aristatus extract decrease in the higher dosage of irradiated sample compared to non-irradiated sample. No presence of scoliosis was observed in all dosage of irradiated and non-irradiated of Orthosiphon aristatus. The heartbeat of zebrafish embryo treated with irradiated Orthosiphon aristatus specifically 12 % show decrease at 250 μg/mL concentration. Remaining dosage of irradiated and non-irradiated Orthosiphon aristatus show average heartbeat around 120-160 bpm. Conclusion: As conclusion, irradiated and non-irradiated of Orthosiphon aristatus is safe to be consumed due to its pharmaceutical effect but it still exhibited mild toxicity effect on zebrafish embryo.
KW - Hatching
KW - Heartbeat
KW - Irradiation
KW - Orthosiphon aristatus
KW - Zebrafish embryo
UR - http://www.scopus.com/inward/record.url?scp=85134002717&partnerID=8YFLogxK
U2 - 10.48048/tis.2022.4980
DO - 10.48048/tis.2022.4980
M3 - Article
AN - SCOPUS:85134002717
SN - 2774-0226
VL - 19
JO - Trends in Sciences
JF - Trends in Sciences
IS - 14
M1 - 4980
ER -