TY - JOUR
T1 - Ethnopharmacological evaluation of selected east kalimantan flora for diabetes therapy
T2 - The isolation of lupane triterpenoids as α-glucosidase inhibitors from ceriops tagal (perr) c.b.robb
AU - Ramadhan, R.
AU - Phuwapraisirisan, P.
AU - Kusuma, I. W.
AU - Amirta, R.
N1 - Publisher Copyright:
© RASĀYAN. All rights reserved.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - The current study evaluated the antidiabetic activity of particular East Kalimantan plants that have long been used by Dayak people for diabetes therapy and identified the active compounds in these plants. However, the antidiabetic activity of the indigenous plants has not been systematically investigated. Phytochemical analysis of six selected East Kalimantan plant extracts was conducted to investigate their: (1) antidiabetic activities (via α-glucosidase inhibition bioassays), (2) DPPH radical scavenging activities, and (3) total antioxidant capacities. Additionally, the mechanisms underlying the inhibitory effects of the isolated compounds were determined by Lineweaver-Burk plots. The extracts showed α-glucosidase inhibitory activities with IC50 values ranging from 0.07 to 8.09 mg/mL. Ceriops tagal exhibited the highest DPPH radical scavenging activity (IC50 26.24 µg/mL), as well as the highest total antioxidant capacity (215.16 ± 0.02 mgGAE/g). Bioassay-guided isolation of C. tagal, which was the plant with the most significant apparent potential, yielded three triterpenoids: lupeol (1), betulone (2), and betulin (3). Of the isolated compounds, 3 exhibited the highest inhibition with IC50 value of 18.87 μM. Furthermore, all isolated triterpenoids showed noncompetitive inhibition action. The systematic screening approaches were successfully applied to identify antidiabetic agents from East Kalimantan plants.
AB - The current study evaluated the antidiabetic activity of particular East Kalimantan plants that have long been used by Dayak people for diabetes therapy and identified the active compounds in these plants. However, the antidiabetic activity of the indigenous plants has not been systematically investigated. Phytochemical analysis of six selected East Kalimantan plant extracts was conducted to investigate their: (1) antidiabetic activities (via α-glucosidase inhibition bioassays), (2) DPPH radical scavenging activities, and (3) total antioxidant capacities. Additionally, the mechanisms underlying the inhibitory effects of the isolated compounds were determined by Lineweaver-Burk plots. The extracts showed α-glucosidase inhibitory activities with IC50 values ranging from 0.07 to 8.09 mg/mL. Ceriops tagal exhibited the highest DPPH radical scavenging activity (IC50 26.24 µg/mL), as well as the highest total antioxidant capacity (215.16 ± 0.02 mgGAE/g). Bioassay-guided isolation of C. tagal, which was the plant with the most significant apparent potential, yielded three triterpenoids: lupeol (1), betulone (2), and betulin (3). Of the isolated compounds, 3 exhibited the highest inhibition with IC50 value of 18.87 μM. Furthermore, all isolated triterpenoids showed noncompetitive inhibition action. The systematic screening approaches were successfully applied to identify antidiabetic agents from East Kalimantan plants.
KW - Antidiabetes
KW - Antioxidant
KW - Ceriops tagal
KW - East Kalimantan
KW - Ethnopharmacology
UR - http://www.scopus.com/inward/record.url?scp=85090638642&partnerID=8YFLogxK
U2 - 10.31788/RJC.2020.1335559
DO - 10.31788/RJC.2020.1335559
M3 - Article
AN - SCOPUS:85090638642
SN - 0974-1496
VL - 13
SP - 1727
EP - 1734
JO - Rasayan Journal of Chemistry
JF - Rasayan Journal of Chemistry
IS - 3
ER -