TY - JOUR
T1 - Enzyme inhibitory activities of marine sponges against cholinesterase and 5α-reductase
AU - Suciati,
AU - Rabgay, Karma
AU - Fachrunniza, Yunda
AU - Saesong, Tongchai
AU - Hadi, Tri Aryono
AU - Wahyuni, Tutik Sri
AU - Widyawaruyanti, Aty
AU - Ingkaninan, Kornkanok
N1 - Publisher Copyright:
© 2019, Malaysian Society of Applied Biology. All rights reserved.
PY - 2019/6
Y1 - 2019/6
N2 - Marine sponges have been the source of various metabolites with potent biological activities. In this study fifteen methanolic extracts of marine sponges, collected off the coast of Tabuhan Island, Banyuwangi, East Java, Indonesia were evaluated in relation to their cholinesterase and 5α-reductase inhibitory activities. The results revealed that the extract of Petrosia sp. inhibited the 5α-reductase enzyme at 100 µg/mL, with 61.21% inhibition, which is slightly lower than the positive control, finasteride, of 76.70%. The results of the cholinesterase inhibitory screening showed that three marine sponges namely, Callyspongia sp., Niphates olemda, and Agelas nakamurai presented notable cholinesterase inhibitory activities. The highest potency was found in A. nakamurai, with an IC50 value of 1.05 µg/mL. All three samples inhibited both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), however, the extract of N. olemda showed a higher inhibition against AChE compared to BuChE. The chemistry of the Callyspongia sp., N. olemda and A. nakamurai were investigated using thin layer chromatography and1H NMR methods. The results suggested the presence of terpenes and alkaloids in the samples. Further study is needed to determine the metabolite responsible for cholinesterase inhibitory activity.
AB - Marine sponges have been the source of various metabolites with potent biological activities. In this study fifteen methanolic extracts of marine sponges, collected off the coast of Tabuhan Island, Banyuwangi, East Java, Indonesia were evaluated in relation to their cholinesterase and 5α-reductase inhibitory activities. The results revealed that the extract of Petrosia sp. inhibited the 5α-reductase enzyme at 100 µg/mL, with 61.21% inhibition, which is slightly lower than the positive control, finasteride, of 76.70%. The results of the cholinesterase inhibitory screening showed that three marine sponges namely, Callyspongia sp., Niphates olemda, and Agelas nakamurai presented notable cholinesterase inhibitory activities. The highest potency was found in A. nakamurai, with an IC50 value of 1.05 µg/mL. All three samples inhibited both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), however, the extract of N. olemda showed a higher inhibition against AChE compared to BuChE. The chemistry of the Callyspongia sp., N. olemda and A. nakamurai were investigated using thin layer chromatography and1H NMR methods. The results suggested the presence of terpenes and alkaloids in the samples. Further study is needed to determine the metabolite responsible for cholinesterase inhibitory activity.
KW - 5α-reductase inhibitor
KW - Acetylcholinesterase inhibitor
KW - Marine sponge
UR - http://www.scopus.com/inward/record.url?scp=85073641463&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85073641463
SN - 0126-8643
VL - 48
SP - 77
EP - 83
JO - Malaysian Applied Biology
JF - Malaysian Applied Biology
IS - 3
ER -