TY - JOUR
T1 - Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method
AU - Rosita, Noorma
AU - Meitasari, Vinta Arnike
AU - Rianti, Mirna Candra
AU - Hariyadi, Dewi Melani
AU - Miatmoko, Andang
N1 - Funding Information:
This work was supported by Universitas Airlangga, Indonesia (grant number 86/UN3/2018, 2018). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
Publisher Copyright:
© 2019 Newlands Press Ltd.
PY - 2019
Y1 - 2019
N2 - Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.
AB - Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.
KW - (-)-epigallocatechin gallate
KW - Span 80
KW - Tween 80
KW - cytotoxicity
KW - partition coefficient
KW - penetration
UR - http://www.scopus.com/inward/record.url?scp=85070193271&partnerID=8YFLogxK
U2 - 10.4155/tde-2019-0015
DO - 10.4155/tde-2019-0015
M3 - Article
C2 - 31319784
AN - SCOPUS:85070193271
SN - 2041-5990
VL - 10
SP - 409
EP - 417
JO - Therapeutic Delivery
JF - Therapeutic Delivery
IS - 7
ER -
