Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method

Noorma Rosita; Vinta Arnike Meitasari; Mirna Candra Rianti; Dewi Melani Hariyadi; Andang Miatmoko

doi:10.4155/tde-2019-0015

Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method

Noorma Rosita, Vinta Arnike Meitasari, Mirna Candra Rianti, Dewi Melani Hariyadi, Andang Miatmoko

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.

Original language	English
Pages (from-to)	409-417
Number of pages	9
Journal	Therapeutic Delivery
Volume	10
Issue number	7
DOIs	https://doi.org/10.4155/tde-2019-0015
Publication status	Published - 2019

Keywords

(-)-epigallocatechin gallate
Span 80
Tween 80
cytotoxicity
partition coefficient
penetration

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4155/tde-2019-0015

Cite this

@article{2f3cd5cb36b94ef2a5176bfadca4d9be,

title = "Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method",

abstract = "Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.",

keywords = "(-)-epigallocatechin gallate, Span 80, Tween 80, cytotoxicity, partition coefficient, penetration",

author = "Noorma Rosita and Meitasari, {Vinta Arnike} and Rianti, {Mirna Candra} and Hariyadi, {Dewi Melani} and Andang Miatmoko",

note = "Publisher Copyright: {\textcopyright} 2019 Newlands Press Ltd.",

year = "2019",

doi = "10.4155/tde-2019-0015",

language = "English",

volume = "10",

pages = "409--417",

journal = "Therapeutic Delivery",

issn = "2041-5990",

publisher = "Taylor and Francis Ltd.",

number = "7",

}

TY - JOUR

T1 - Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method

AU - Rosita, Noorma

AU - Meitasari, Vinta Arnike

AU - Rianti, Mirna Candra

AU - Hariyadi, Dewi Melani

AU - Miatmoko, Andang

PY - 2019

Y1 - 2019

N2 - Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.

AB - Aim: (-)-Epigallocatechin gallate (EGCG) has been reported as inducing apoptosis in cervical cancer. However, EGCG demonstrates low skin permeability. In order to develop topical delivery of EGCG, the partition coefficient, log P, was modified using a reverse micelle method. Results & methodology: During this study, Tween 80 and Span 80 were added to EGCG at hydrophilic-lipophilic balance (HLB) values of 4.3, 6 and 8. The results showed that lowering the HLB value increases the log P value of EGCG and results in higher IC50 values in Henrietta Lacks (HeLa) cancer cells than that of EGCG. Surfactant-modified EGCG-HLB 6 produced faster and deeper skin penetration than EGCG. Conclusion: Modification of log P value using a combination of Tween 80 and Span 80 improved cytotoxicity and topical delivery of EGCG.

KW - (-)-epigallocatechin gallate

KW - Span 80

KW - Tween 80

KW - cytotoxicity

KW - partition coefficient

KW - penetration

UR - http://www.scopus.com/inward/record.url?scp=85070193271&partnerID=8YFLogxK

U2 - 10.4155/tde-2019-0015

DO - 10.4155/tde-2019-0015

M3 - Article

C2 - 31319784

AN - SCOPUS:85070193271

SN - 2041-5990

VL - 10

SP - 409

EP - 417

JO - Therapeutic Delivery

JF - Therapeutic Delivery

IS - 7

ER -

Enhancing skin penetration of epigallocatechin gallate by modifying partition coefficient using reverse micelle method

Abstract

Keywords

UN SDGs

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