TY - JOUR
T1 - Enhancement of osteogenesis using a combination of hydroxyapatite and stem cells from exfoliated deciduous teeth
AU - Prahasanti, Chiquita
AU - Krismariono, Agung
AU - Takanamita, Rifiana
AU - Wijaksana, I. Komang Evan
AU - Suardita, Ketut
AU - Saskianti, Tania
AU - Ernawati, Diah Savitri
N1 - Funding Information:
The authors would like to thank the Faculty of Dental Medicine, Universitas Airlangga and Dr. Soetomo General Hospital Surabaya. This research was supported by Universitas Airlangga.
Publisher Copyright:
© 2020 University of Dicle.
PY - 2020
Y1 - 2020
N2 - Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve with the result that alternative therapies for achieving periodontal regeneration are still lacking. Tissue engineering using stem cells and biomaterial scaffold represents an alternative for solving this problem. Stem cells from human exfoliated deciduous teeth (SHED) are one source of adult stem cells which can proliferate and differentiate into many types of specific cells. Hydroxyapatite (HA) constitutes a bioactive scaffold possessing osseointegration, osteoconduction and osteogenesis properties when used as bone graft. The aim of this study was to analyze osteocalcin (OCN) and osteopontin (OPN) expression after the application of a combination of HA and SHED to alveolar bone defects. Eight experimental animals were divided into two groups. The first group was transplanted with HA only, while the second was transplanted with HA/SHED. The expression of OCN and OPN was examined 21 days later by means of immunohistochemistry analysis. The analysis result of an independent T-test with significance value p= 0,0041 < 0.05 showed a significant difference between the two groups. The HA/SHED group showed a significantly higher expression of OCN and OPN than that of the HA only group. Transplantation of a combination of HA and SHED onto alveolar defects increases the expression of OCN and OPN.
AB - Periodontal regeneration, especially regeneration of alveolar bone, remains difficult to achieve with the result that alternative therapies for achieving periodontal regeneration are still lacking. Tissue engineering using stem cells and biomaterial scaffold represents an alternative for solving this problem. Stem cells from human exfoliated deciduous teeth (SHED) are one source of adult stem cells which can proliferate and differentiate into many types of specific cells. Hydroxyapatite (HA) constitutes a bioactive scaffold possessing osseointegration, osteoconduction and osteogenesis properties when used as bone graft. The aim of this study was to analyze osteocalcin (OCN) and osteopontin (OPN) expression after the application of a combination of HA and SHED to alveolar bone defects. Eight experimental animals were divided into two groups. The first group was transplanted with HA only, while the second was transplanted with HA/SHED. The expression of OCN and OPN was examined 21 days later by means of immunohistochemistry analysis. The analysis result of an independent T-test with significance value p= 0,0041 < 0.05 showed a significant difference between the two groups. The HA/SHED group showed a significantly higher expression of OCN and OPN than that of the HA only group. Transplantation of a combination of HA and SHED onto alveolar defects increases the expression of OCN and OPN.
KW - Hydroxyapatite
KW - Osteocalcin
KW - Osteopontin
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=85090891011&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85090891011
SN - 1309-100X
VL - 13
SP - 508
EP - 512
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -