Enhancement of EPA, DHA and Quality of Commercial Feed by the Addition of Papain and Phytase Enzyme in Nile Tilapia (Oreochromis niloticus)

Fish feed is important in intensive cultivation of nile tilapia. The purpose of this research is to know the effect and the optimum dose of the combination of papain enzyme and phytase enzyme on quality of commercial feed, EPA and DHA of Nile Tilapia. The combination of papain enzyme and phytase enzyme in feed influenced dry matter, crude protein, crude lipid, nitrogen-free extracts phosphorous and in EPA and DHA commercial feed, in nile tilapia, but had no effect on crude fiber and ash content. Dose of 1% papain enzyme and 0,05% phytase enzyme (P1) was the best to increase crude protein, however 3% papain enzyme and 0,1% phytase enzyme (P2) has decreased the nitrogen-free extracts and increased the phosphorous, while P3 was the best to increase, EPA and DHA in nile tilapia.