Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

Aamir Shehzad; Christijogo Sumartono; Jusak Nugraha; Helen Susilowati; Andi Yasmin Wijaya; Hafiz Ishfaq Ahmad; Wiwiek Tyasningsih; Fedik Abdul Rantam

doi:10.33640/2405-609X.3221

Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

Aamir Shehzad, Christijogo Sumartono, Jusak Nugraha, Helen Susilowati, Andi Yasmin Wijaya, Hafiz Ishfaq Ahmad, Wiwiek Tyasningsih, Fedik Abdul Rantam

Research output: Contribution to journal › Article › peer-review

Abstract

The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (þ). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.

Original language	English
Pages (from-to)	275-291
Number of pages	17
Journal	Karbala International Journal of Modern Science
Volume	8
Issue number	2
DOIs	https://doi.org/10.33640/2405-609X.3221
Publication status	Published - 2022

Keywords

Bioinformatics
MHC-I and MHC-II
Multi-epitope vaccine
Public health
SARS-CoV-2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.33640/2405-609X.3221

Cite this

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title = "Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach",

abstract = "The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a ({\th}). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.",

keywords = "Bioinformatics, MHC-I and MHC-II, Multi-epitope vaccine, Public health, SARS-CoV-2",

author = "Aamir Shehzad and Christijogo Sumartono and Jusak Nugraha and Helen Susilowati and Wijaya, {Andi Yasmin} and Ahmad, {Hafiz Ishfaq} and Wiwiek Tyasningsih and Rantam, {Fedik Abdul}",

note = "Funding Information: The current study was funded by the Research Centre for Vaccine Technology and Development, Institute of Tropical Disease (RCVTD-ITD) at Universitas Airlangga Surabaya, Indonesia. All authors would like to thank the Institute of Tropical Diseases (ITD) experts for their technical assistance. Publisher Copyright: {\textcopyright} 2022 University of Kerbala.",

year = "2022",

doi = "10.33640/2405-609X.3221",

language = "English",

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pages = "275--291",

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TY - JOUR

T1 - Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

AU - Shehzad, Aamir

AU - Sumartono, Christijogo

AU - Nugraha, Jusak

AU - Susilowati, Helen

AU - Wijaya, Andi Yasmin

AU - Ahmad, Hafiz Ishfaq

AU - Tyasningsih, Wiwiek

AU - Rantam, Fedik Abdul

N1 - Funding Information: The current study was funded by the Research Centre for Vaccine Technology and Development, Institute of Tropical Disease (RCVTD-ITD) at Universitas Airlangga Surabaya, Indonesia. All authors would like to thank the Institute of Tropical Diseases (ITD) experts for their technical assistance. Publisher Copyright: © 2022 University of Kerbala.

PY - 2022

Y1 - 2022

N2 - The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (þ). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.

AB - The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (þ). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.

KW - Bioinformatics

KW - MHC-I and MHC-II

KW - Multi-epitope vaccine

KW - Public health

KW - SARS-CoV-2

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M3 - Article

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SN - 2405-609X

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SP - 275

EP - 291

JO - Karbala International Journal of Modern Science

JF - Karbala International Journal of Modern Science

IS - 2

ER -

Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

Abstract

Keywords

UN SDGs

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