Encoding gene for Methicillin Resistant Staphylococcus aureus (MRSA) isolated from nasal swab of dogs

Reina Puspita Rahmaniar; Maya Nurwartanti Yunita; Mustofa Helmi Effendi; Sheila Marty Yanestria

Encoding gene for Methicillin Resistant Staphylococcus aureus (MRSA) isolated from nasal swab of dogs

Reina Puspita Rahmaniar, Maya Nurwartanti Yunita, Mustofa Helmi Effendi, Sheila Marty Yanestria

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from five areas in Surabaya. Bacterial identification was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA confirmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identification on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health.

Original language	English
Pages (from-to)	37-40
Number of pages	4
Journal	Indian Veterinary Journal
Volume	97
Issue number	2
Publication status	Published - 2020

Keywords

Dogs
MRSA
MecA gene
Staphylococcus aureus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from five areas in Surabaya. Bacterial identification was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA confirmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identification on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health.",

keywords = "Dogs, MRSA, MecA gene, Staphylococcus aureus",

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T1 - Encoding gene for Methicillin Resistant Staphylococcus aureus (MRSA) isolated from nasal swab of dogs

AU - Rahmaniar, Reina Puspita

AU - Yunita, Maya Nurwartanti

AU - Effendi, Mustofa Helmi

AU - Yanestria, Sheila Marty

PY - 2020

Y1 - 2020

N2 - The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from five areas in Surabaya. Bacterial identification was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA confirmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identification on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health.

AB - The purpose of this study was to isolate and identify encoding gene for methicillin-resistant Staphylococcus aureus (MRSA) from nasal swab of dogs in Surabaya, Indonesia. Nasal swab of dogs of 85 samples obtained from five areas in Surabaya. Bacterial identification was based on the growth in Mannitol Salt Agar, Gram staining, catalase, coagulase and VP tests. 43 (50.59%) out of 85 samples were for positive Staphylococcus aureus isolation. MRSA confirmation by Oxacillin Resistant Screen Agar Base (ORSAB) were 25(29.41%). The molecular identification on mecA gene by PCR showed that 5(5.88%) isolates were positive contain mecA gene. It was concluded that the dogs as companion animals can be a potential reservoir for MRSA strains to threat public health.

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KW - MRSA

KW - MecA gene

KW - Staphylococcus aureus

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JO - Indian Veterinary Journal

JF - Indian Veterinary Journal

IS - 2

ER -

Encoding gene for Methicillin Resistant Staphylococcus aureus (MRSA) isolated from nasal swab of dogs

Abstract

Keywords

UN SDGs

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