Introduction: Skin aging, particularly photoaging, is a widely experienced problem. The therapies for photoaging are continuously evolving. For example, the application of topical antioxidants, such as vitamin C, has been widely used with satisfactory outcomes. Stem cell therapy, including amniotic membrane stem cell-conditioned medium (AMSC-CM), is a promising alternative because it contains growth factors and cytokines that aid wound healing and skin rejuvenation. The aim of this study was to evaluate the efficacy of AMSC-CM and vitamin C combination therapy following CO2 fractional laser as laser-assisted drug delivery for photoaging treatment. Methods: This was an analytical experimental study conducted on 30 patients with photoaged skin treated with AMSC-CM and vitamin C. The treatment was repeated three times with an interval of 4 weeks. The final evaluation included Janus skin analysis to assess wrinkles, pores, spots, and skin tone. Results: Among the 30 participants with photoaged skin who met the inclusion criteria, the mean age was 50.53 (± 5.39) years and the mean Glogau score was 2.77 (± 0.43). The treatment resulted in significant improvements (P <.05) on wrinkles from evaluation I to evaluations II and evaluation I to evaluations III, pores from evaluation I to evaluation II, and spots from evaluation I to evaluation II. Conclusion: Clinical improvements were observed on the participants’ photoaged skin in terms of wrinkles, pores, and spots after the combined topical therapy of AMSC-CM and vitamin C following CO2 fractional laser therapy.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalMalaysian Journal of Medicine and Health Sciences
Publication statusPublished - Sept 2021


  • Amniotic membrane stem cell-conditioned medium
  • Laser fractional CO
  • Photoaging
  • Vitamin C


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