Effects of Vitamin C and E Supplementation with High-Intensity Swimming on Bone Adaption in Mice

The significance of physical activity has increased in recent years, as evidenced by the rise in participation levels High-intensity exercise can be harmful to bone health if not done correctly. This study investigates the effect of high-intensity exercise mixed with vitamin C and supplementation on bone cellular plasticity, with the goal of discovering new techniques to improve skeletal integrity. A study was performed on 28 female mice (Mus musculus) that were healthy, not pregnant, and were between 3-4 months. The mice weighed between 18-35 grams. The mice were allocated into four groups through a random process: a control group (CON), which did not receive any treatment; a group that received supplementation of vitamins C and E (VIT); a group that underwent high-intensity swimming exercise (EXC); and a combined treatment group that received both vitamin supplementation and high-intensity exercise (CBD). After 28 days, femur bones were gathered for histological examination in order to quantify the amount of osteoblasts, osteocytes, osteoclasts, and cortex thickness. The EXC group had the fewest osteoblasts (25.9±4.7) and osteocytes (26.9±4.7), which suggests that the intense exercise caused a lot of bone breakdown. In contrast, the CBD group, had markedly larger numbers of osteoblasts (37.9±3.6) and osteocytes (54.7±7.2), while having the lowest count of osteoclasts (0.7 ± 0.2). The statistical analysis showed significant disparities in the number of osteocytes and osteoblasts between the EXC group and both the VIT and CBD groups (p<0.05). Intense activity in mice leads to an increase in bone resorption, which may negatively affect bone health. Supplementation with vitamins C and E has been shown to have a preventive effect by promoting bone formation. Additional investigation is necessary to examine the fundamental processes and lasting consequences.