Effects of methotrexate, Moringa oleifera, and Andrographis paniculata extracts on the myocardial and aortic tissue of streptozotocin-nicotinamide-induced hyperglycemic rats

Dimas Bathoro Bagus Pamungkas, Viskasari Pintoko Kalanjati, Abdurachman, Dwi Martha Nur Aditya, Muhammad Husni Fansury Nasution, Maya Rahmayanti Syamhadi

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND Methotrexate (MTX) could lower glucose levels in type 1 diabetes mellitus, while Moringa oleifera and Andrographis paniculata supplementations have similar effects on hyperglycemia. This study aimed to analyze the effects of MTX, M. oleifera, and A. paniculata leaf extracts on the myocardial interleukin (IL)-6 and the histopathology of the left ventricle and aorta. METHODS 49 rats were divided equally into 7 groups: negative control and diabetic induced by streptozotocin-nicotinamide (STZ-NA) injection consisting of positive control (STZ-NA only), M. oleifera (500 mg/kgBW/day), A. paniculata (500 mg/kgBW/ day), MTX (7 mg/kgBW/week), MTX (7 mg/kgBW/week)+M. oleifera (500 mg/kgBW/ day), and MTX (7 mg/kgBW/week)+A. paniculata (500 mg/kgBW/day). We analyzed oral MTX, M. oleifera, and A. paniculata leaf extracts’ effects on random blood glucose, myocardial IL-6, and cardiac histopathology of STZ-NA-induced hyperglycemic male rats. Data were analyzed using Wilcoxon and Kruskal–Wallis tests. RESULTS Myocardial IL-6 in the M. oleifera group was significantly lower compared to the positive control group (p = 0.041). Compared to the positive control group, the myocardial necrosis and aortic intima–media thickness in the MTX+A. paniculata group were significantly reduced (p = 0.005 and 0.001, respectively). CONCLUSIONS MTX, M. oleifera, and A. paniculata showed antihyperglycemic effect, both individually and in combination. A. paniculata leaf extract had a significant cardioprotective effect in STZ-NA-induced hyperglycemia.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalMedical Journal of Indonesia
Volume32
Issue number3
DOIs
Publication statusPublished - Sept 2023

Keywords

  • Andrographis paniculata
  • Moringa oleifera
  • cardiovascular disease
  • diabetes
  • methotrexate

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