Menopausal condition with low estrogen level caused decreased in glycogen synthesis. Glycogen had a role in maintaining glucose level between 60 and 140 mg/dL and also for supplying energy of cells. Glycogen made up 5 % of liver volume more than in skeletal and muscle (2%).This research was aimed to determine the effects of leaf ethanol extract of Graptophyllum pictum L. Griff. to promote liver glycogen synthesis of ovariectomized mice. It is used 24 female mice divided into six group, namely group G1, G2 and G3 were control group and group G4,G5 and G6 were treatment groups that were given leaf ethanol extract of Graptophyllum pictum L. Griff. with dosis 10 mg/kg, 20 mg/kg and 30 mg/kg. In G1 the mice used were not ovariectomized and G2 until G6, all mice were ovariectomized. The treatment was done for 6 week orally and then all mice were measured for the serum glucose level by Glucometer. Then the all mice were anesthetized and sacrificed to take the liver for measuring liver glycogen level by colorimetric. The result showed that the leaf ethanol extract of Graptophyllum pictum L.Griff could increase glycogen synthesis shown by decreasing of glucose level and increasing glycogen level. And its optimal dosis was 10 mg/kg.