Effects of Lactobacillus plantarum IS-10506 on gastric protective mucosal factors: an ibuprofen-induced gastric mucosal injury

Andy Darma, Wibi Riawan, Khadijah Rizky Sumitro, Alpha Fardah Athiyyah, Reza Ranuh, Mohammad Fathul Qorib, Ingrid Surono, Subijanto Marto Sudarmo

Research output: Contribution to journalArticlepeer-review


Background: Gastric mucosal protective factor plays an important roles in gastric homeostasis between mucosal injury and mucosal repair. Previous study implicated probiotics in the repair of the intestinal mucosa, while probiotic role in gastric mucosa remains poorly understood. This study aims to determine the role of probiotic L. plantarum IS-10506 in gastric protective mucosal factors by examining the expression of cyclooxygenase-1 (COX-1), COX-2, prostaglandin E2 (PGE2), and gastric mucus production (Mucin 5AC/ MUC5AC) by surface mucous cells as gastric mucosal protective factors. Methods: Twenty-seven male Wistar rats (Rattus norwegicus) were divided into 3 groups, ie. control, curative and preventive group. Ibuprofen 300mg/kgBW were given to all groups to induce gastric injury, while Lactobacillus plantarum IS-10506 1x109 CFU/day were given into the curative (5 days) and preventive (12 days) groups. Antrum tissue with primary monoclonal antibodies againts COX-1, COX-2, PGE2, and MUC5AC were examined in the day-1,-3, and-5 after necropsy. Results: Number of cells expressed COX-1, COX-2, PGE2 and MUC5AC in curative and preventive groups were significantly higher than control group. L. plantarum IS-10506 showed its efficacy in gastric mucosal repair on third day in the curative group and on first day in the preventive group. L. plantarum IS-10506 probiotic plays a role in gastric protective mucosal factors by promoting cell expression of COX-1, PGE2, and MUC5AC. In addition, it has been determined that L. plantarum IS-10506 inhibits COX-2 expression, that may lead to minimalize the inflammatory conditions and a deterioration of the gastric mucosal environment. Conclusion: Lactobacillus plantarum IS-10506 can prevent ibuprofen-induced antral mucosal damage through gastric protective mucosal factors.

Original languageEnglish
Pages (from-to)514-518
Number of pages5
JournalBali Medical Journal
Issue number1
Publication statusPublished - 2023


  • COX-1
  • COX-2
  • Lactobacillus plantarum IS-10506
  • MUC5AC
  • PGE2
  • gastric


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