Effects Leaf Ethanol Extract of Graptophyllum pictum L. Griff. to Inhibit Vaginal Atrophy of Menopausal Mouse

Menopausal condition with low estrogen level caused vaginal atrophy that it is a condition where vaginal lining become
dryer and thinner and it would be easier to get inflammation. So, it was important to find out the treatment to inhibit it.
This research was aimed to determine the effects of Graptophyllum pictum (L.) Griff. leaf ethanol extract to inhibit
vaginal atrophy of menopausal mice. It used 24 female mice divided into six groups, namely, Group 1, 2 and 3 as control
group and group 4,5 and 6 as the treatment groups that were given leaf ethanol extract of Graptophyllum pictum L.Griff.
with dozes 10 mg/kg, 20 mg/kg, and 30 mg/kg. Menopausal mice were obtained by doing ovariectomy in mice. Six
weeks after the treatment, all mice were anesthetized and dissected to take the vagina for slide preparation staining with
PAS staining, IHC for GLTU1 and for measuring the glycogen content. The data were the epithelium thickness and
glycogen expression, GLTU 1 expression and glycogen level of menopausal mice vagina. All data were analyzed by
Anova test, and the result showed that {Graptophyllum pictum (L.) Griff} leaf ethanol extract effected significantly on
the vaginal epithelium thickness and glycogen expression, GLTU1 expression and glycogen level of ovariectomized
mice, so it could inhibit vaginal atrophy. The optimal dozes were 10 mg/kg its leaf extract.