TY - JOUR
T1 - Effectivity of Insulin Transferrin Selenium and Bovine Serum Albumin addition on in vitro culture medium on fertilization and blastocyst rate of mice (Mus musculus)
AU - Widjiati,
AU - Luqman, Epy Muhammad
AU - Tehupuring, Benjamin Christoffel
N1 - Publisher Copyright:
© 2018 Journal of International Dental and Medical Research.
PY - 2017
Y1 - 2017
N2 - The aim of this research was to know best composition of in vitro culture medium that can support the development of zygote and cleavage into blastocyst stage of embryo and ready to be transferred into recipient. Insulin Transferrin Selenium (ITS) and Bovine Serum Albumin (BSA) need to be added to optimize culture medium so it can produce embryo with high viability to support embryo transfer program. The addition of Insulin Transferrin Selenium into in vitro culture medium bind free radical, trigger development of the cell, inhibit damage of the cell because of its antioxidant component so it can increase blastocyst viability. The addition of Bovine Serum Albumin can increase the competence of the embryo development to grow in the in vitro culture medium. The research started with estrus synchronized, oocyte collection, in vitro fertilization, addition of Insulin Transferrin Selenium and Bovine Serum Albumin into culture medium and examine the number of fertilization and blastocyst. The result show combination of Insulin Transferrin Selenium and Bovine Serum Albumin supplementation increasing number of fertilization and blastocyst is better compare with group that added Insulin Transferrin Selenium only (p > 0,05) but it is not different with group that added Bovine Serum Albumin only. The conclusion from this research is addition of Insulin Transferrin Selenium and Bovine Serum Albumincan increase the number of fertilization and support the development of embryo.
AB - The aim of this research was to know best composition of in vitro culture medium that can support the development of zygote and cleavage into blastocyst stage of embryo and ready to be transferred into recipient. Insulin Transferrin Selenium (ITS) and Bovine Serum Albumin (BSA) need to be added to optimize culture medium so it can produce embryo with high viability to support embryo transfer program. The addition of Insulin Transferrin Selenium into in vitro culture medium bind free radical, trigger development of the cell, inhibit damage of the cell because of its antioxidant component so it can increase blastocyst viability. The addition of Bovine Serum Albumin can increase the competence of the embryo development to grow in the in vitro culture medium. The research started with estrus synchronized, oocyte collection, in vitro fertilization, addition of Insulin Transferrin Selenium and Bovine Serum Albumin into culture medium and examine the number of fertilization and blastocyst. The result show combination of Insulin Transferrin Selenium and Bovine Serum Albumin supplementation increasing number of fertilization and blastocyst is better compare with group that added Insulin Transferrin Selenium only (p > 0,05) but it is not different with group that added Bovine Serum Albumin only. The conclusion from this research is addition of Insulin Transferrin Selenium and Bovine Serum Albumincan increase the number of fertilization and support the development of embryo.
KW - Blastosis rate
KW - Cultur medium
KW - Fertilization rate
KW - In vitro fertilization
UR - http://www.scopus.com/inward/record.url?scp=85050259720&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85050259720
SN - 1309-100X
VL - 10
SP - 1080
EP - 1083
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 3
ER -