TY - JOUR
T1 - Effectiveness of purple leaves (Graptophyllum pictum L. Griff) and hydroxyapatite as socket preservation biomaterials
AU - Sitalaksmi, Ratri Maya
AU - Azharpratomo, Rizkipriyanto
AU - Irsan, Shafira Ninditya
AU - Rahmania, Primanda Nur
AU - Wati, Sisca Meida
AU - Adam, Syafira Salsabila
AU - Bilbalqish, Kavanila
AU - Shariff, Khairul Anuar bin
AU - Laksono, Harry
AU - Dahlan, Agus
AU - Rianti, Devi
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2025/1/1
Y1 - 2025/1/1
N2 - Background: Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation. Aim: To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition. Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21. Results: The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21. Conclusion: The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21.
AB - Background: Tooth extraction without socket preservation will lead to reduction in the dimensions and volume of the alveolar bone. Bone defects resulting from tooth extraction not only hinder prosthetic reconstruction but also present aesthetic issues and complicating dental implant treatment. Purple leaves contains flavonoids, steroids, tannins, saponins,and non-toxic alkaloids. These compounds play roles as antimicrobials, immunomodulators, antioxidants, anti-inflammatories, analgesics. Hydroxyapatite has good biocompatibility, and can induce osteoblast differentiation. Aim: To investigate the effects of the combining nanoemulsion extract of purple leaves and hydroxyapatite on the expression of RUNX2, OSX, OPN, ALP, and calcium deposition. Method: This research used MTT Assay, ICC, and Alizarin Red staining. The study groups were as follows: Group 1: Ad-MSCs; Group 2: Ad-MSCs + αmem; Group 3: Ad-MSCs + osteogenic medium + 1 % nano-extract of Purple leaves & Hydroxyapatite; Group 4: Ad-MSCs + osteogenic medium + 2 % nano-extract of Purple leaves & Hydroxyapatite. Observations were conducted on days 7, 14, and 21. Results: The combination of nanosuspension extract of Purple leaves and hydroxyapatite significantly increased the expression of RUNX2, OSX, OPN,ALP and calcium deposition compared to other groups. The combination of nanoemulsion extract of Purple leaves and hydroxyapatite were significant (P < 0.05) compared to the control group on each day 7, day 14 and day 21. Conclusion: The combination of nanosuspension extract of Purple leaves and hydroxyapatite was able to enhance the expression of RUNX2, OSX, OPN, ALP, and calcium deposition on days 7,14,21.
KW - Bone regeneration
KW - Hydroxyapatite
KW - Purple leaves
KW - Socket preservation
UR - http://www.scopus.com/inward/record.url?scp=85211118876&partnerID=8YFLogxK
U2 - 10.1016/j.jobcr.2024.12.002
DO - 10.1016/j.jobcr.2024.12.002
M3 - Article
AN - SCOPUS:85211118876
SN - 2212-4268
VL - 15
SP - 63
EP - 68
JO - Journal of Oral Biology and Craniofacial Research
JF - Journal of Oral Biology and Craniofacial Research
IS - 1
ER -