Effect of vitamin D during orthodontic tooth movement on receptor activator of nuclear factor Kappa-B ligand expression and osteoclast number in pregnant wistar rat (Rattus novergicus)

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Abstract

Background: Female patients have the possibility to become pregnant during orthodontic treatment. Vitamin D usually consumed by pregnant women. Estrogen and Vitamin D could affect bone metabolism. Aim and Objectives: The aim of this study was to analyze the effect of vitamin D during orthodontic movement in pregnant rats by Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) expression and osteoclast number. Material and Methods: The experimental observational analytic study with post-test only control group design and simple random sampling method was conducted. 24-healthy-female Wistar rats were divided into 4 groups; K1: pregnant rats with orthodontic tooth movement and vitamin D on Day 7; K2: pregnant rats with orthodontic tooth movement and vitamin D on Day 14; K3: pregnant rats with orthodontic tooth movement without vitamin D on Day 7 and; K4: pregnant rats with orthodontic tooth movement without vitamin D on Day 14. Nickle-Titanium coil spring with 10 g/mm2 force was placed between the incisors and the maxillary molars. The RANKL expression and osteoclasts number were analyzed using Analysis of Variance (ANOVA) (p<0.05). Results: The highest osteoclasts number (8.494 ± 1.194), and RANKLexpression (7.967 ± 2.185) found in K1 group with significant between groups (p<0.05).Conclusions: Vitamin D increase osteoclast number and RANKL expression during orthodontic tooth movement in pregnant rats.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalJournal of Krishna Institute of Medical Sciences University
Volume8
Issue number1
Publication statusPublished - 2019

Keywords

  • Orthodontic tooth movement
  • Osteoclast
  • Pregnant
  • Receptor activator of nuclear factor kappa-B Ligand
  • Vitamin D

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