TY - JOUR
T1 - Effect of Vitamin C and E Supplementation and Combination of Both in Egg Yolk Tris Diluter on the Quality of Sapera Goat Spermatozoa in the 5 °C Cooling Process
AU - Saputro, Amung Logam
AU - Syadid, Uliy Ba sin
AU - Rimayanti,
AU - Susilowati, Suherni
AU - Prastiya, Ragil Angga
AU - Agustono, Bodhi
AU - Mumtazi, Fauzan
AU - Marifatunnisa'romadhona,
AU - Anastasya,
AU - Farhan, Muhammad Riesta
N1 - Publisher Copyright:
© 2022 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2022/9
Y1 - 2022/9
N2 - This study was aimed to determine the effect of vitamin C, E supplementation and both combination in the egg yolk tris diluent on the quality of Sapera goat spermatozoa in 5℃ cooling process. The samples were fresh semen collected from Sapera goat. It was divided into four different treatment groups. The control treatment or P0 contained semen sample + egg yolk tris diluent, P1 contained semen sample + egg yolk tris diluent + vitamin C 0,9 mg/ml, P2 contained semen sample + egg yolk tris diluent + vitamin E 1 mg/ml, P3 contained semen sample + egg yolk tris diluent + vitamin C 0,9 mg/ml + vitamin E 1 mg/ml. The least decrease of spermatozoa motility, viability and membrane integrity was found in P3, which was significantly different to P0 (p<0,05). However, P1 and P2 did not show significant difference in the results of motility and viability evaluation (p>0,05). It can be concluded that the combination of vitamin C and E supplementation can be an optimal antioxidants supplement in egg yolk tris diluent on the quality of Sapera goat spermatozoa in 5℃ cooling process.
AB - This study was aimed to determine the effect of vitamin C, E supplementation and both combination in the egg yolk tris diluent on the quality of Sapera goat spermatozoa in 5℃ cooling process. The samples were fresh semen collected from Sapera goat. It was divided into four different treatment groups. The control treatment or P0 contained semen sample + egg yolk tris diluent, P1 contained semen sample + egg yolk tris diluent + vitamin C 0,9 mg/ml, P2 contained semen sample + egg yolk tris diluent + vitamin E 1 mg/ml, P3 contained semen sample + egg yolk tris diluent + vitamin C 0,9 mg/ml + vitamin E 1 mg/ml. The least decrease of spermatozoa motility, viability and membrane integrity was found in P3, which was significantly different to P0 (p<0,05). However, P1 and P2 did not show significant difference in the results of motility and viability evaluation (p>0,05). It can be concluded that the combination of vitamin C and E supplementation can be an optimal antioxidants supplement in egg yolk tris diluent on the quality of Sapera goat spermatozoa in 5℃ cooling process.
KW - 5℃ Cooling Process
KW - Quality of Spermatozoa
KW - Sapera Goat
KW - Vitamin C
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=85144531684&partnerID=8YFLogxK
U2 - 10.5530/pj.2022.14.150
DO - 10.5530/pj.2022.14.150
M3 - Article
AN - SCOPUS:85144531684
SN - 0975-3575
VL - 14
SP - 661
EP - 665
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 5
ER -