Effect of using oestrus or non-oestrus teaser cows on ejaculation time and cortisol level in blood of madura bulls during semen collection

Ejaculation time was measured when semen collection was done and blood samples were estimated using Indirect ELISA for determining the effect of using oestrus and non oestrus teaser cow on ejaculation time and blood cortisol levels as an indicator of stress of Madura bulls in Madura, Indonesia. The ejaculation time differed significantly (p <0.05) between using teaser cow in oestrus (1.975 ± 0.499 minutes) and non-oestrus (2.994 ± 0.528 minutes). There was no significant difference between cortisol level of bulls when using teaser cow in oestrus (11.920 ± 4.169 ng/ml) and non-oestrus (9.803 ± 4.989 ng/ml) during semen collection.