TY - JOUR
T1 - Effect of temperature on sex and steroid hormones of purple mud crab, Scylla tranquebarica (Fabricius, 1798) during egg incubation, larvae rearing and juvenile production
AU - Hidir, Ariffin
AU - Aaqillah-Amr, Mohd Amran
AU - Mohd-Sabri, Muda
AU - Mohd-Zaidi, Ibrahim
AU - Shahreza, Md Sheriff
AU - Abualreesh, Muyassar H.
AU - Peng, Teoh Hong
AU - Ma, Hongyu
AU - Waiho, Khor
AU - Fazhan, Hanafiah
AU - Mukti, Akhmad Taufiq
AU - Ikhwanuddin, Mhd
N1 - Funding Information:
The study was funded by Golden Goose Research Grant, Universiti Malaysia Terengganu (Vot. No. 55189). The study is also part of the research program under the Higher Institution Center of Excellence (HICoE) grant for the development of future food through sustainable shellfish aquaculture (HICoE AKUATROP Trust Account No. 66955). The authors are grateful to all the staff at the Institute of Tropical Aquaculture and Fisheries. Special thanks to Megat Fadhlul Hazmi and Redzuari Alias for the larval rearing consultation.
Publisher Copyright:
© 2022 John Wiley & Sons Ltd.
PY - 2022/8
Y1 - 2022/8
N2 - The influence of temperature on sex determination has been reported previously on many crustaceans. The interpretability of temperature capability to regulate the steroidogenic pathway, which in turn governs the offspring sex of mud crab, still remains unclear. Thus, the aim was to determine the effects of temperature on sex percentage and steroidal pathway in purple mud crab, Scylla tranquebarica. In sex percentage analysis, the eggs (28°C and 30°C), larvae (28°C, 30°C and 32°C) and juveniles (26°C, 28°C, 30°C) were exposed to different temperatures. The results revealed that exposure to a higher temperature during larvae (32°C) and juvenile (30°C) stages probably initiated the maleness (58.7%) and femaleness (60.8%) mechanism respectively. In steroid hormone analysis, the eggs (26°C, 28°C, 30°C and 32°C), larvae (29°C, 30°C, 31°C and 32°C) and juveniles (26°C, 28°C, 30°C and 32°C) were exposed to different temperatures. The steroidal assay corroborated the results of the sex percentages, which found higher dihydrotestosterone (172.4 pg/g) and testosterone (580.2 pg/g) levels when the larvae were incubated at a higher temperature (32°C) while higher estrone (136.9 pg/g) and estradiol (540.7 pg/g) levels when the juveniles were reared at high temperature (30°C). In brief, the temperature plays a significant role to redirect the mud crab sex during the larval and juvenile stages.
AB - The influence of temperature on sex determination has been reported previously on many crustaceans. The interpretability of temperature capability to regulate the steroidogenic pathway, which in turn governs the offspring sex of mud crab, still remains unclear. Thus, the aim was to determine the effects of temperature on sex percentage and steroidal pathway in purple mud crab, Scylla tranquebarica. In sex percentage analysis, the eggs (28°C and 30°C), larvae (28°C, 30°C and 32°C) and juveniles (26°C, 28°C, 30°C) were exposed to different temperatures. The results revealed that exposure to a higher temperature during larvae (32°C) and juvenile (30°C) stages probably initiated the maleness (58.7%) and femaleness (60.8%) mechanism respectively. In steroid hormone analysis, the eggs (26°C, 28°C, 30°C and 32°C), larvae (29°C, 30°C, 31°C and 32°C) and juveniles (26°C, 28°C, 30°C and 32°C) were exposed to different temperatures. The steroidal assay corroborated the results of the sex percentages, which found higher dihydrotestosterone (172.4 pg/g) and testosterone (580.2 pg/g) levels when the larvae were incubated at a higher temperature (32°C) while higher estrone (136.9 pg/g) and estradiol (540.7 pg/g) levels when the juveniles were reared at high temperature (30°C). In brief, the temperature plays a significant role to redirect the mud crab sex during the larval and juvenile stages.
KW - egg
KW - juvenile
KW - larvae
KW - mud crab
KW - sex hormone
KW - temperature
UR - http://www.scopus.com/inward/record.url?scp=85130328606&partnerID=8YFLogxK
U2 - 10.1111/are.15911
DO - 10.1111/are.15911
M3 - Article
AN - SCOPUS:85130328606
SN - 1355-557X
VL - 53
SP - 4095
EP - 4105
JO - Aquaculture Research
JF - Aquaculture Research
IS - 11
ER -