TY - GEN
T1 - Effect of sucrose, erythrose-4-phosphate and phenylalanine on biomassa and flavonoid content of callus culture from leaves of Gynura procumbens Merr.
AU - Nurisa, Aryana
AU - Kristanti, Alfinda Novi
AU - Manuhara, Yosephine Sri Wulan
N1 - Publisher Copyright:
© 2017 Author(s).
PY - 2017/8/4
Y1 - 2017/8/4
N2 - The aims of this study were to know the effect of concentration of sucrose, erythrose-4-phosphate and phenylalanine on biomass and flavonoid content of callus cultures from leaves of sambung nyawa (Gynura procumbens Merr.). This study was experimental research with complete randomized design. Callus induction was treated in MS medium supplemented with NAA 2 mg/L, BAP 1 mg/L and sucrose concentration (10 g/L, 30 g/L and 50 g/L) respectively were combined with erythrose-4-phosphate (0 μM, 2,5 μM and 5 μM) and phenylalanine (0 mg/L, 2 mg/L and 3 mg/L), each treatment were repeated four times. After six weeks of culture, fresh and dry weight of calli were measured and extracted with ethanol absolut. Crude extract ethanolic of callus was analyzed used by a modified colorimetric with spectrophotometer method. The best yield of calli biomass (0,672 ± 0,112 gram of fresh weight and 0,033 ± 0,009 gram of dry weight) was obtained in treatment of 30 g/L sucrose of and 5 μM erythrose-4-phosphate. The highest total flavonoid content was obtained of calli treated with 30 g/L of sucrose and 3 mg/L of phenylalanine (3633,4 ppm quercetin/gram dry weight and 15777,8 ppm kaempferol/gram dry weight).
AB - The aims of this study were to know the effect of concentration of sucrose, erythrose-4-phosphate and phenylalanine on biomass and flavonoid content of callus cultures from leaves of sambung nyawa (Gynura procumbens Merr.). This study was experimental research with complete randomized design. Callus induction was treated in MS medium supplemented with NAA 2 mg/L, BAP 1 mg/L and sucrose concentration (10 g/L, 30 g/L and 50 g/L) respectively were combined with erythrose-4-phosphate (0 μM, 2,5 μM and 5 μM) and phenylalanine (0 mg/L, 2 mg/L and 3 mg/L), each treatment were repeated four times. After six weeks of culture, fresh and dry weight of calli were measured and extracted with ethanol absolut. Crude extract ethanolic of callus was analyzed used by a modified colorimetric with spectrophotometer method. The best yield of calli biomass (0,672 ± 0,112 gram of fresh weight and 0,033 ± 0,009 gram of dry weight) was obtained in treatment of 30 g/L sucrose of and 5 μM erythrose-4-phosphate. The highest total flavonoid content was obtained of calli treated with 30 g/L of sucrose and 3 mg/L of phenylalanine (3633,4 ppm quercetin/gram dry weight and 15777,8 ppm kaempferol/gram dry weight).
UR - http://www.scopus.com/inward/record.url?scp=85028343745&partnerID=8YFLogxK
U2 - 10.1063/1.4995205
DO - 10.1063/1.4995205
M3 - Conference contribution
AN - SCOPUS:85028343745
T3 - AIP Conference Proceedings
BT - 4th International Conference on Research, Implementation, and Education of Mathematics and Science, 4TH ICRIEMS
A2 - Suyanto, Slamet
A2 - Kusumawardani, Cahyorini
A2 - Wilujeng, Insih
A2 - Abadi, Agus Maman
PB - American Institute of Physics Inc.
T2 - 4th International Conference on Research, Implementation, and Education of Mathematics and Sciences: Research and Education for Developing Scientific Attitude in Sciences and Mathematics, ICRIEMS 2017
Y2 - 15 May 2017 through 16 May 2017
ER -