Effect of Silicone Rubber/Polyvinyl Alcohol /Zirconium Oxide Compound on Mesenchymal Stromal Cells

Objective: This study aimed to conduct a cytotoxicity testindeterminingtheviabilityandproliferationprofile fornovelmaterialofsiliconerubber/polyvinylalcohol (PVA)withorwithoutZirconiumoxideonmesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2x10⁵seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statisticalanalysis was conducted using ANOVA, or the Kruskal-Walli’s test with a CI of 95 %. Results: After exposure to silicone rubber/PVA+ZrO₂ material, the viability ofmesenchymalstromalcells was significantly lower in Silicone rubber/PVA+ZrO₂ 3 % (p< 0.05), compared to Silicone rubber/PVA+ZrO₂ 5 % (90,998 ± 3,970 vs. 107,762 ± 7,892). The percentage of living cells from mesenchymal stromal cell cultures after exposure to silicone rubber/PVA+ZrO₂ day-1 was not statistically significant, but silicone rubber/ PVA had the maximum percentage (102.47 %). In contrast to day 1, the results of the ANOVA test on days -3 and -5 revealed a significant difference between the 5 groups (p<0.001). Similarly, the Tukey-Kramer post-hoc test on the group yielded comparable results. Decreased across all groups were observed on day 5 of observation with 3 % ZrO₂ group being the lowest. Conclusion: Silicone rubber/polyvinyl alcohol (PVA)compoundwithorwithoutZirconium oxide (ZrO₂) exposure did not show a toxic effect on mesenchymal stroma cell culture. Further, in vivo studies are needed to confirm our findings.