Effect of rSLPI amnion membrane application on incision wound of rattus norvegicus in collagen and VEGF expression

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Amniotic membrane is one of the natural biomaterial that is widely used for wound healing which has Secretory Leukocyte Protease Inhibitor (SLPI) as an active component. One of rSLPI functions is suppressing several pro-inflammatory factors, such as serine protease. Supression of serine proteinase in wound healing process may increase the amount of vascular endothelial growth factor (VEGF) and collagen. The aim of this study is to evaluate the effect of rSLP amniotic membrane fluid application on incision wound of Rattus norvegicus in expression of VEGF and collagen. The rats divided into 4 groups: control group and treated group with rSLPI (P1 0.03 cc, P2 0,045 cc, and P3 0.06 cc) (n=6). Insicion was made on rat's cervical back. On the day 4, tissues around insicion wound are collected for Masson's Trychrome (MT) and Immunohistochemistry (IHC) analysis. Expression of VEGF and collagen are calculated using microscope with 400x magnification. Kruskal-Wallis test of VEGF showed significant value with p=0,006 (p < 0.05). Mann-Whitney test of VEGF showed a significant difference between control and treatment group. Oneway ANOVA test result for collagen has p value 0.000 (p < 0.05). Honestly Significant Difference (HSD) test showed a significant differences between all group. Application of rSLPI amniotic membran fluid in Rattus norvegicus incision wound may increase VEGF and collagen.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalJournal of International Dental and Medical Research
Issue number2
Publication statusPublished - 2017


  • Amniotic membrane
  • Collagen
  • VEGF


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