TY - JOUR
T1 - Effect of rSLPI amnion membrane application on incision wound of rattus norvegicus in collagen and VEGF expression
AU - Munadziroh, Elly
AU - Soekartono, R. Helal
AU - Rafli, Rossa Bella Vennowusky
AU - Yuliati, Anita
AU - Kartikasari, Nadia
PY - 2017
Y1 - 2017
N2 - Amniotic membrane is one of the natural biomaterial that is widely used for wound healing which has Secretory Leukocyte Protease Inhibitor (SLPI) as an active component. One of rSLPI functions is suppressing several pro-inflammatory factors, such as serine protease. Supression of serine proteinase in wound healing process may increase the amount of vascular endothelial growth factor (VEGF) and collagen. The aim of this study is to evaluate the effect of rSLP amniotic membrane fluid application on incision wound of Rattus norvegicus in expression of VEGF and collagen. The rats divided into 4 groups: control group and treated group with rSLPI (P1 0.03 cc, P2 0,045 cc, and P3 0.06 cc) (n=6). Insicion was made on rat's cervical back. On the day 4, tissues around insicion wound are collected for Masson's Trychrome (MT) and Immunohistochemistry (IHC) analysis. Expression of VEGF and collagen are calculated using microscope with 400x magnification. Kruskal-Wallis test of VEGF showed significant value with p=0,006 (p < 0.05). Mann-Whitney test of VEGF showed a significant difference between control and treatment group. Oneway ANOVA test result for collagen has p value 0.000 (p < 0.05). Honestly Significant Difference (HSD) test showed a significant differences between all group. Application of rSLPI amniotic membran fluid in Rattus norvegicus incision wound may increase VEGF and collagen.
AB - Amniotic membrane is one of the natural biomaterial that is widely used for wound healing which has Secretory Leukocyte Protease Inhibitor (SLPI) as an active component. One of rSLPI functions is suppressing several pro-inflammatory factors, such as serine protease. Supression of serine proteinase in wound healing process may increase the amount of vascular endothelial growth factor (VEGF) and collagen. The aim of this study is to evaluate the effect of rSLP amniotic membrane fluid application on incision wound of Rattus norvegicus in expression of VEGF and collagen. The rats divided into 4 groups: control group and treated group with rSLPI (P1 0.03 cc, P2 0,045 cc, and P3 0.06 cc) (n=6). Insicion was made on rat's cervical back. On the day 4, tissues around insicion wound are collected for Masson's Trychrome (MT) and Immunohistochemistry (IHC) analysis. Expression of VEGF and collagen are calculated using microscope with 400x magnification. Kruskal-Wallis test of VEGF showed significant value with p=0,006 (p < 0.05). Mann-Whitney test of VEGF showed a significant difference between control and treatment group. Oneway ANOVA test result for collagen has p value 0.000 (p < 0.05). Honestly Significant Difference (HSD) test showed a significant differences between all group. Application of rSLPI amniotic membran fluid in Rattus norvegicus incision wound may increase VEGF and collagen.
KW - Amniotic membrane
KW - Collagen
KW - RSLPI
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=85027440777&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85027440777
SN - 1309-100X
VL - 10
SP - 239
EP - 243
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -