Effect of Nocturnal and Diurnal Moderate-intensity Swimming Exercise on Increasing Irisin Level of Female Mice (Mus musculus)

Muhamad Fauzi Antoni, Purwo Sri Rejeki, Sulistiawati, Adi Pranoto, Kristanti Wanito Wigati, Gadis Meinar Sari, Ronny Lesmana, Yoshio Yamaoka

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4 Citations (Scopus)

Abstract

This study aims to compare moderate-intensity swimming exercise in the morning and at night towards the increment of irisin levels in female mice (Mus musculus). This research is a real experiment with the randomized control group post-test-only design. A total of 24 female mice (Mus musculus), aged 3 months old, and weighing 20-40 grams (Lee’s Index Value > 0.3) were randomized into three groups, namely G1 (n = 8, control without intervention), G2 (n = 8, moderate-intensity swimming exercise in the morning), and G3 (n = 8, moderate-intensity swimming exercise at night). Swimming exercises were carried out 3x/w for 4 weeks with an intensity of 6% of body weight and the duration was 70% of maximum swimming time. Irisin levels were measured using the ELISA method. The data analysis techniques used were one-way ANOVA test and Tukey’s HSD post-hoc test. The results that were obtained from this experimental study were mean levels of irisin in G1 (1.86 ± 0.06 ng/mL), G2 (2.66 ± 0.12 ng/mL), G3 (3.43 ± 0.35 ng/mL), and (P≤ 0.001). The results of Tukey’s HSD post-hoc test showed that there was a significant difference in the mean post-training irisin levels between G2 and G1 (P≤ 0.05), G3 and G1 (P≤ 0.001), and G3 and G2 (P≤ 0.05). Based on the results of this study, it was concluded that moderate-intensity swimming exercises in the morning and at night were able to increase irisin levels. However, moderate-intensity swimming exercise at night showed a higher effectiveness in increasing irisin levels of female mice (Mus musculus).

Original languageEnglish
Article numbere2022033
JournalChiang Mai University Journal of Natural Sciences
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 2022

Keywords

  • Irisin levels
  • Morning exercise
  • Night exercise
  • Obesity

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