TY - JOUR
T1 - Effect of Nocturnal and Diurnal Moderate-intensity Swimming Exercise on Increasing Irisin Level of Female Mice (Mus musculus)
AU - Antoni, Muhamad Fauzi
AU - Rejeki, Purwo Sri
AU - Sulistiawati,
AU - Pranoto, Adi
AU - Wigati, Kristanti Wanito
AU - Sari, Gadis Meinar
AU - Lesmana, Ronny
AU - Yamaoka, Yoshio
N1 - Publisher Copyright:
© 2022
PY - 2022/4
Y1 - 2022/4
N2 - This study aims to compare moderate-intensity swimming exercise in the morning and at night towards the increment of irisin levels in female mice (Mus musculus). This research is a real experiment with the randomized control group post-test-only design. A total of 24 female mice (Mus musculus), aged 3 months old, and weighing 20-40 grams (Lee’s Index Value > 0.3) were randomized into three groups, namely G1 (n = 8, control without intervention), G2 (n = 8, moderate-intensity swimming exercise in the morning), and G3 (n = 8, moderate-intensity swimming exercise at night). Swimming exercises were carried out 3x/w for 4 weeks with an intensity of 6% of body weight and the duration was 70% of maximum swimming time. Irisin levels were measured using the ELISA method. The data analysis techniques used were one-way ANOVA test and Tukey’s HSD post-hoc test. The results that were obtained from this experimental study were mean levels of irisin in G1 (1.86 ± 0.06 ng/mL), G2 (2.66 ± 0.12 ng/mL), G3 (3.43 ± 0.35 ng/mL), and (P≤ 0.001). The results of Tukey’s HSD post-hoc test showed that there was a significant difference in the mean post-training irisin levels between G2 and G1 (P≤ 0.05), G3 and G1 (P≤ 0.001), and G3 and G2 (P≤ 0.05). Based on the results of this study, it was concluded that moderate-intensity swimming exercises in the morning and at night were able to increase irisin levels. However, moderate-intensity swimming exercise at night showed a higher effectiveness in increasing irisin levels of female mice (Mus musculus).
AB - This study aims to compare moderate-intensity swimming exercise in the morning and at night towards the increment of irisin levels in female mice (Mus musculus). This research is a real experiment with the randomized control group post-test-only design. A total of 24 female mice (Mus musculus), aged 3 months old, and weighing 20-40 grams (Lee’s Index Value > 0.3) were randomized into three groups, namely G1 (n = 8, control without intervention), G2 (n = 8, moderate-intensity swimming exercise in the morning), and G3 (n = 8, moderate-intensity swimming exercise at night). Swimming exercises were carried out 3x/w for 4 weeks with an intensity of 6% of body weight and the duration was 70% of maximum swimming time. Irisin levels were measured using the ELISA method. The data analysis techniques used were one-way ANOVA test and Tukey’s HSD post-hoc test. The results that were obtained from this experimental study were mean levels of irisin in G1 (1.86 ± 0.06 ng/mL), G2 (2.66 ± 0.12 ng/mL), G3 (3.43 ± 0.35 ng/mL), and (P≤ 0.001). The results of Tukey’s HSD post-hoc test showed that there was a significant difference in the mean post-training irisin levels between G2 and G1 (P≤ 0.05), G3 and G1 (P≤ 0.001), and G3 and G2 (P≤ 0.05). Based on the results of this study, it was concluded that moderate-intensity swimming exercises in the morning and at night were able to increase irisin levels. However, moderate-intensity swimming exercise at night showed a higher effectiveness in increasing irisin levels of female mice (Mus musculus).
KW - Irisin levels
KW - Morning exercise
KW - Night exercise
KW - Obesity
UR - http://www.scopus.com/inward/record.url?scp=85129858923&partnerID=8YFLogxK
U2 - 10.12982/CMUJNS.2022.033
DO - 10.12982/CMUJNS.2022.033
M3 - Article
AN - SCOPUS:85129858923
SN - 1685-1994
VL - 21
JO - Chiang Mai University Journal of Natural Sciences
JF - Chiang Mai University Journal of Natural Sciences
IS - 2
M1 - e2022033
ER -