Effect of Nigella sativa extract on the elevation of serum MDA levels in aluminum-induced oxidative stress in rats

Aluminum (Al) constitutes approximately 8% of the Earth's surface. Al is extensively used in various industrial applications, including medicines, food additives, cosmetics and farming practices. However, Al poses a risk to human health when it enters the body, potentially leading to various pathological conditions. Nigella sativa, known for its active compound thymoquinone (TQ), has demonstrated anti-inflammatory and antioxidant properties. TQ is recognized for its potential in the prevention and treatment of numerous diseases. This study aimed to explore the effect of N. sativa extract (NSE) in preventing the elevation of malondialdehyde (MDA) levels in the blood serum of rats subjected to aluminum-induced oxidative stress. Twenty-four male Wistar rats were divided into four groups. Groups K1 and K2 were fed a standard diet, while groups K3 and K4 received NSE at doses 200 mg/kg/day and 400 mg/kg/day, respectively, administrated through oral gavage. After two weeks of NSE pretreatment, groups K2, K3, and K4 were exposed to AlCl3 (34 mg/kg, oral gavage) for an additional three weeks. There were no significant differences (P > 0.05) in body weight or hemoglobin levels among the groups. Interestingly, a significant reduction in MDA level (P < 0.05) was observed among groups. Notably, MDA levels were lower in the NSE-treated and control groups compared to the AlCl3-exposed group. In conclusion, NSE may have a positive effect against oxidative stress induced by Al exposure.