Effect of NaOH-based deproteinisation on chemical and biological properties of Moina sp. chitosan

W. Tjahjaningsih; R. S.P. Pitutur; A. S. Mubarak; M. A.A. Husein; S. Raseetha; M. G.P. Elkavanto; M. R. Rachmansyah; F. Z. Hanif

doi:10.26656/fr.2017.7(S4).1

Effect of NaOH-based deproteinisation on chemical and biological properties of Moina sp. chitosan

W. Tjahjaningsih, R. S.P. Pitutur, Patmawati, A. S. Mubarak, M. A.A. Husein, S. Raseetha, M. G.P. Elkavanto, M. R. Rachmansyah, F. Z. Hanif

Research output: Contribution to journal › Article › peer-review

Abstract

Chitosan is a biopolymer which is isolated from the crustacean exoskeleton of Moina sp. with particular antibacterial properties. Alkali-sodium hydroxide deproteinisation has a huge role in ensuring the chitosan quality by removing protein to enhance its dispersivity. Modification of sodium hydroxide (NaOH) concentration by 3N and 6N was performed in this study to observe the effect on chemical and biological characteristics of chitosan. The highest concentration treatment of 6N NaOH exhibited the best composition of protein, fat, and ash by 6.09%, 3.77% and 0.9%, respectively. The deacetylation degree of chitosan treated with 3N NaOH depicted the highest solubility of 73.25% compared to the solubility of 6N NaOH by 61.06%. The antibacterial activity of Moina sp. chitosan was considered as having a weak and fluctuated activity by different concentrations of NaOH. The 6N NaOH treatment chitosan showed the best antibacterial activity against the gram-positive bacterial strain of Staphylococcus aureus with the widest inhibition zone diameter of 4.34±0.55 mm, while the 3N NaOH treatment chitosan showed the best antibacterial activity against the gram-negative bacterial strain of Escherichia coli with widest inhibition zone diameter of 4.47±0.78 mm.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Food Research
Volume	7
DOIs	https://doi.org/10.26656/fr.2017.7(S4).1
Publication status	Published - 2023

Keywords

Chitosan
Deproteinization
Fisheries
Moina
Sustainable yield

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10.26656/fr.2017.7(S4).1

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title = "Effect of NaOH-based deproteinisation on chemical and biological properties of Moina sp. chitosan",

abstract = "Chitosan is a biopolymer which is isolated from the crustacean exoskeleton of Moina sp. with particular antibacterial properties. Alkali-sodium hydroxide deproteinisation has a huge role in ensuring the chitosan quality by removing protein to enhance its dispersivity. Modification of sodium hydroxide (NaOH) concentration by 3N and 6N was performed in this study to observe the effect on chemical and biological characteristics of chitosan. The highest concentration treatment of 6N NaOH exhibited the best composition of protein, fat, and ash by 6.09%, 3.77% and 0.9%, respectively. The deacetylation degree of chitosan treated with 3N NaOH depicted the highest solubility of 73.25% compared to the solubility of 6N NaOH by 61.06%. The antibacterial activity of Moina sp. chitosan was considered as having a weak and fluctuated activity by different concentrations of NaOH. The 6N NaOH treatment chitosan showed the best antibacterial activity against the gram-positive bacterial strain of Staphylococcus aureus with the widest inhibition zone diameter of 4.34±0.55 mm, while the 3N NaOH treatment chitosan showed the best antibacterial activity against the gram-negative bacterial strain of Escherichia coli with widest inhibition zone diameter of 4.47±0.78 mm.",

keywords = "Chitosan, Deproteinization, Fisheries, Moina, Sustainable yield",

author = "W. Tjahjaningsih and Pitutur, {R. S.P.} and Patmawati and Mubarak, {A. S.} and Husein, {M. A.A.} and S. Raseetha and Elkavanto, {M. G.P.} and Rachmansyah, {M. R.} and Hanif, {F. Z.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors.",

year = "2023",

doi = "10.26656/fr.2017.7(S4).1",

language = "English",

volume = "7",

pages = "1--9",

journal = "Food Research",

issn = "2550-2166",

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TY - JOUR

T1 - Effect of NaOH-based deproteinisation on chemical and biological properties of Moina sp. chitosan

AU - Tjahjaningsih, W.

AU - Pitutur, R. S.P.

AU - Patmawati,

AU - Mubarak, A. S.

AU - Husein, M. A.A.

AU - Raseetha, S.

AU - Elkavanto, M. G.P.

AU - Rachmansyah, M. R.

AU - Hanif, F. Z.

PY - 2023

Y1 - 2023

N2 - Chitosan is a biopolymer which is isolated from the crustacean exoskeleton of Moina sp. with particular antibacterial properties. Alkali-sodium hydroxide deproteinisation has a huge role in ensuring the chitosan quality by removing protein to enhance its dispersivity. Modification of sodium hydroxide (NaOH) concentration by 3N and 6N was performed in this study to observe the effect on chemical and biological characteristics of chitosan. The highest concentration treatment of 6N NaOH exhibited the best composition of protein, fat, and ash by 6.09%, 3.77% and 0.9%, respectively. The deacetylation degree of chitosan treated with 3N NaOH depicted the highest solubility of 73.25% compared to the solubility of 6N NaOH by 61.06%. The antibacterial activity of Moina sp. chitosan was considered as having a weak and fluctuated activity by different concentrations of NaOH. The 6N NaOH treatment chitosan showed the best antibacterial activity against the gram-positive bacterial strain of Staphylococcus aureus with the widest inhibition zone diameter of 4.34±0.55 mm, while the 3N NaOH treatment chitosan showed the best antibacterial activity against the gram-negative bacterial strain of Escherichia coli with widest inhibition zone diameter of 4.47±0.78 mm.

AB - Chitosan is a biopolymer which is isolated from the crustacean exoskeleton of Moina sp. with particular antibacterial properties. Alkali-sodium hydroxide deproteinisation has a huge role in ensuring the chitosan quality by removing protein to enhance its dispersivity. Modification of sodium hydroxide (NaOH) concentration by 3N and 6N was performed in this study to observe the effect on chemical and biological characteristics of chitosan. The highest concentration treatment of 6N NaOH exhibited the best composition of protein, fat, and ash by 6.09%, 3.77% and 0.9%, respectively. The deacetylation degree of chitosan treated with 3N NaOH depicted the highest solubility of 73.25% compared to the solubility of 6N NaOH by 61.06%. The antibacterial activity of Moina sp. chitosan was considered as having a weak and fluctuated activity by different concentrations of NaOH. The 6N NaOH treatment chitosan showed the best antibacterial activity against the gram-positive bacterial strain of Staphylococcus aureus with the widest inhibition zone diameter of 4.34±0.55 mm, while the 3N NaOH treatment chitosan showed the best antibacterial activity against the gram-negative bacterial strain of Escherichia coli with widest inhibition zone diameter of 4.47±0.78 mm.

KW - Chitosan

KW - Deproteinization

KW - Fisheries

KW - Moina

KW - Sustainable yield

UR - http://www.scopus.com/inward/record.url?scp=85178486239&partnerID=8YFLogxK

U2 - 10.26656/fr.2017.7(S4).1

DO - 10.26656/fr.2017.7(S4).1

M3 - Article

AN - SCOPUS:85178486239

SN - 2550-2166

VL - 7

SP - 1

EP - 9

JO - Food Research

JF - Food Research

ER -

Effect of NaOH-based deproteinisation on chemical and biological properties of Moina sp. chitosan

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Keywords

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