EFFECT OF MUCOADHESIVE GINGIVAL PATCH FROM MANGOSTEEN PERICARP (GARCINIA MANGOSTANA L.) EXTRACT ON THE SOD LEVELS AND THE NUMBER OF NECROTIC CELLS DUE TO PANORAMIC RADIOGRAPHY RADIATION IN ACUTE PHASE

Panoramic radiography is a low-dose x-ray radiation technique often used in dentistry to determine the diagnosis, treatment plans and monitoring the results of treatments. It has ionizing radiation effect which cause free radical formation. The increase of free radicalactivity in cells can cause tissue damage by increasing peroxidation lipid process and resulting in necrosis. Superoxide Dismutase (SOD) as an endogenous antioxidant produced by the body to reduce free radicals has not been able to prevent cell death. Mangosteen’s pericarp (Garcinia mangostana) contains xanthone as a bioactive component that has a high level of antioxidants. This study utilizes mucoadhesive gingival patches containing mangosteen’s pericarp extract that facilitate the absorption of the bioactive substance in the gingival tissue that were exposed by radiation. The aim of this study is to determine the effect of mangosteen pericarp’s mucoadhesive gingival patch on the SOD levels and necrotics cells number in gingival tissues due to panoramic radiography radiation. Thirty-five Rattus norvegicus samples were divided into 7 groups, given with mucoadhesive gingival patches and were exposed with x-ray radiation. The gingival tissues were taken for histopathological examination to determine the necrotic cell number and ELISA test to measure SOD levels. There were differences in SOD levels and necrotic cell number in gingival tissue between rats that were given mangosteen gingival patches and those that were not. Application of mangosteen’s pericarp mucoadhesive patches can increase the SOD levels and decrease the number of necrotic cells in rat’s gingival tissues due to panoramic radiography radiation in acute phase.