Effect of Malus sylvestris extract on histopathological features of hypercholesterolemic wistar rat (Rattus norvegicus) fatty liver

Objective: To evaluate the effect of Malus sylvestris extract on improving the degree of steatosis and portal inflammation histopathological features of hypercholesterolemic Rattus norvegicus strain wistar. Method: Forty-two male rats were divided into 6 groups randomly. Hypercholesterolemic fatty liver induced by giving high-fat diet (HFD) for 46 days on groups 1 to 5, while group 6 given standard diet with the same amount of time. Simvastatin was administered in group 2 at a dose of 0.36 mg/day. Groups 3, 4, and 5 were given Malus sylvestris extract (MSE) as a treatment at doses of 90 mg/day, 180 mg/day, and 360 mg/day in sequential order. Simvastatin and MSE were administered for 14 days, from day 33 to 46. On day 47, all rats were sacrificed and the liver was removed for histopathological slides preparation with hematoxylin-eosin (HE) staining. Histopathological results were analyzed using Kruskal Wallis test followed by Mann Whitney test. Result: Histopathological analysis showed that Malus sylvestris extract improved steatosis and portal inflammation features compared to HFD-fed rats in group 1 (p<0,05). Conclusion: Malus sylvestris extract improved the degree of steatosis and portal inflammation histopathological features of hypercholesterolemic rat fatty liver.