TY - JOUR
T1 - Effect of hydroxyapatite coating thickness on inflammation and osseointegration of Ti–29Nb–13Ta-4.6Zr (TNTZ) implants
AU - Nuswantoro, Nuzul Ficky
AU - Gunawarman,
AU - Manjas, Menkher
AU - Suharti, Netti
AU - Juliadmi, Dian
AU - Kasuma, Nila
AU - Yusuf, Yusril
AU - Aminatun,
AU - Sari, Yessie Widya
AU - Niinomi, Mitsuo
AU - Akahori, Toshikazu
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/5/1
Y1 - 2024/5/1
N2 - The study investigates the effect of Hydroxyapatite (HA) coating thickness on the inflammation and osseointegration properties of Ti–29Nb–13Ta-4.6Zr (TNTZ) implants for orthopedic implant applications. TNTZ screws were used as based samples, and the HA coating was deposited using an Electrophoretic Deposition (EPD) method to achieve various coating thickness. The thin (50–70 μm), medium (70–90 μm), and thick (90–110 μm) coating thicknesses were obtained by applying different voltage and time exposure during the EPD process, while the uncoated screw was used as the control sample. The screws were implanted into the right tibia of male Rattus norvegicus Wistar rats, and the animals were kept in cages and provide with ad libitum food for two weeks. After termination of the test animals, blood samples were collected to measure levels of Tumor Necrosis Factor-α (TNF-α) using an ELISA method, and a removal torque test was performed on the implanted screws. Additionally, tibial bone tissue around the implant area was collected for histopathology analysis. The result of the study indicate that HA coating thickness has an effect on TNF-α concentration, removal torque value, and new bone growth. The optimal thickness of the HA layer was found to be in the medium range (70–90 μm), resulting in low inflammation levels, relatively high osseointegration rates, and optimal new bone tissue growth. These finding are expected to be applicable in future orthopedic implant applications.
AB - The study investigates the effect of Hydroxyapatite (HA) coating thickness on the inflammation and osseointegration properties of Ti–29Nb–13Ta-4.6Zr (TNTZ) implants for orthopedic implant applications. TNTZ screws were used as based samples, and the HA coating was deposited using an Electrophoretic Deposition (EPD) method to achieve various coating thickness. The thin (50–70 μm), medium (70–90 μm), and thick (90–110 μm) coating thicknesses were obtained by applying different voltage and time exposure during the EPD process, while the uncoated screw was used as the control sample. The screws were implanted into the right tibia of male Rattus norvegicus Wistar rats, and the animals were kept in cages and provide with ad libitum food for two weeks. After termination of the test animals, blood samples were collected to measure levels of Tumor Necrosis Factor-α (TNF-α) using an ELISA method, and a removal torque test was performed on the implanted screws. Additionally, tibial bone tissue around the implant area was collected for histopathology analysis. The result of the study indicate that HA coating thickness has an effect on TNF-α concentration, removal torque value, and new bone growth. The optimal thickness of the HA layer was found to be in the medium range (70–90 μm), resulting in low inflammation levels, relatively high osseointegration rates, and optimal new bone tissue growth. These finding are expected to be applicable in future orthopedic implant applications.
KW - Coating thickness
KW - Hydroxyapatite
KW - Inflammation
KW - Osseointegration
KW - TNTZ
UR - http://www.scopus.com/inward/record.url?scp=85192458135&partnerID=8YFLogxK
U2 - 10.1016/j.jmrt.2024.05.013
DO - 10.1016/j.jmrt.2024.05.013
M3 - Article
AN - SCOPUS:85192458135
SN - 2238-7854
VL - 30
SP - 6210
EP - 6217
JO - Journal of Materials Research and Technology
JF - Journal of Materials Research and Technology
ER -