TY - JOUR
T1 - Effect of Glutaraldehyde Concentration Variation on Diclofenac Sodium Scaffolds as Cross-Linking Agent
AU - Budiatin, Aniek Setiya
AU - Su’aida, Nily
AU - Lamakluang, Aziszia Insanya
AU - Rahma, Silda Sabila
AU - Zulkarnain, Bambang Subakti
AU - Isadiartuti, Dewi
N1 - Funding Information:
1. International Joint Symposium The 8th Asia Pasific Pharmacy Education Network (APPEN) and the 2th The Halal Pharmaceuticals and Cosmetics (HPC) 2. Indonesian governmental institution of research, technology, and higher education for funding this research.
Publisher Copyright:
© RJPT All right reserved.
PY - 2022/11
Y1 - 2022/11
N2 - Chitosan and gelatin were used as polymer scaffolds for cartilage tissue engineering. The scaffold was used as a biodegradable drug delivery system for diclofenac sodium to treat cartilage defects on osteoarthritis (OA). The materials were composed of diclofenac sodium, chitosan, gelatin, and cross-linking agent-glutaraldehyde (GTA) were form as scaffold. The purpose of this study to investigate the effect of GTA concentration variations (0.00%; 0.25%; 0.50%; 1.00%; 2.50%) on characteristics and the release of diclofenac sodium from chitosan-gelatin scaffold. The scaffolds were made by using the pre-freezing method with a temperature of-56 ± 5°C for 24 hours and characterized by porosity, pore size, swelling, degradation, toxicity test, and diclofenac sodium released from chitosan-gelatin scaffolds at pH and temperature body. The results showed, the addition of GTA increased the swelling ratio from 195.79 ± 7.04% to 793.49 ± 6.92% and minimized weight loss up to 50.98 ± 0.82%, percentage of living cells >60%, optimal porosity at 106.94 ± 9.38 % with pore size 135.48 ± 89.70 µm, diclofenac sodium as sustained release drug completed in 542 hours and the release was following zero-order kinetic. Chitosan-gelatin scaffold is a potential candidate for cartilage tissue engineering and drug delivery system for diclofenac sodium.
AB - Chitosan and gelatin were used as polymer scaffolds for cartilage tissue engineering. The scaffold was used as a biodegradable drug delivery system for diclofenac sodium to treat cartilage defects on osteoarthritis (OA). The materials were composed of diclofenac sodium, chitosan, gelatin, and cross-linking agent-glutaraldehyde (GTA) were form as scaffold. The purpose of this study to investigate the effect of GTA concentration variations (0.00%; 0.25%; 0.50%; 1.00%; 2.50%) on characteristics and the release of diclofenac sodium from chitosan-gelatin scaffold. The scaffolds were made by using the pre-freezing method with a temperature of-56 ± 5°C for 24 hours and characterized by porosity, pore size, swelling, degradation, toxicity test, and diclofenac sodium released from chitosan-gelatin scaffolds at pH and temperature body. The results showed, the addition of GTA increased the swelling ratio from 195.79 ± 7.04% to 793.49 ± 6.92% and minimized weight loss up to 50.98 ± 0.82%, percentage of living cells >60%, optimal porosity at 106.94 ± 9.38 % with pore size 135.48 ± 89.70 µm, diclofenac sodium as sustained release drug completed in 542 hours and the release was following zero-order kinetic. Chitosan-gelatin scaffold is a potential candidate for cartilage tissue engineering and drug delivery system for diclofenac sodium.
KW - Chitosan
KW - Diclofenac Sodium
KW - Drug Delivery System
KW - Gelatin
KW - Glutaraldehyde
KW - Scaffold
UR - http://www.scopus.com/inward/record.url?scp=85144646536&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2022.00836
DO - 10.52711/0974-360X.2022.00836
M3 - Article
AN - SCOPUS:85144646536
SN - 0974-3618
VL - 15
SP - 4974
EP - 4980
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 11
ER -