This study was aimed to evaluate the in vitro toxicity of ethinyl estradiol (EE2) in different concentrations (0; 10; 15; 25; 50 IU/mL) using sperm cells of model organism tropical fish, Barbodes binotatus. Sperm quality parameters, including mass and individual time of sperm motility (second), viability (%), and sperm velocity (μm/s) were measured by digital inverted microscope and DNA fragmentation of sperm after exposure to EE2 was examined with fluorescence microscopy.Barbodes binotatus sperm was collected by stripping then exposed to EE2. The results showed that EE2 exposure could negatively affect some sperm quality parameters, which might significantly reduce the mass and individual time of sperm motility, sperm viability and sperm velocity rate of this animal. DNA fragmentation in sperm of B. binotatus was increased after EE2 exposure compared to the control group. We conclude that administration of EE2 concentration 10 IU/mL decreased mass and individual motility time, velocity, viability of sperm and increased DNA fragmentation in sperm of B. binotatus.
|Number of pages||9|
|Publication status||Published - 1 Jul 2018|
- Barbodes binotatus ethinyl estradiol
- Sperm quality