Effect of erythropoietin administration on spermatogonium amount, sertoli cell and leydig on rats testis (Wistar strain) after vas deferens ligation released

The study aimed to determine the effect of Erythropoietin on spermatogonia number, Sertoli cells and Leydig cells in white rat Wistar strain testis after ligation vas deferens release. Twenty-four Wistar strain eats were grouped into four group. The control group only performed an orchiectomy for testicular examination, ligation group vas deferens, group performed release ligation of vas deferens and group performed release ligation of vas deferens and given EPO injection with dose of 1000 iu/kg BW intraperitoneally for a week (three times per week). The observation of spermatogonium, Sertoli cells and Leydig cells by determined amount on 5 cross sections of seminiferous tubules used 400x light magnificent microscope with Hematoxylin Eosin staining. The result showed ligation of vas deferens had significant decreased spermatogonia number and Sertoli cells (p<0.05). There was no significant difference Leydig cells number after ligation of vas deferens (p>0.05). Meanwhile, release ligation of vas deferens had no significant different in spermatogonia, Sertoli cells and Leydig cells with ligation of vas deferens group. Besides, treatment ligation of vas deferens release and an EPO injection for a week also no significant different in number compared ligation release group of vas deferens.