Effect of different endometriosis implant origin on the expression of estrogen receptor-α, estrogen receptor-β, and progesterone receptor in mice model of endometriosis

Purpose: The main objectives of this study were to reveal the different expression of estrogen receptor (ER)-α, ER-β, and progesterone receptor (PR) in mice model of endometriosis receiving endometrial implants derived from three different origins. Materials and Methods: Female mice (Mus musculus) 32 individuals were injected with 0.2 cc cyclosporin A for each mouse. Mice were divided into three groups with 10 replicates per treatment. As much as, 0.1 ml supernatant of each endometriosis origin was injected into the peritoneal cavities of mice. On day 15, mice were sacrificed and dissected. Expression of ER-α, ER-β, and PR had been examined by immunohistochemistry. Data were analyzed using the SPSS for Windows 19.0. Results: The results of the study, based on immunoreactive score (IRS), demonstrated that mice group which had been injected with adenomyosis tissue (Group C) had the highest average of IRS and had the strongest expression of ER-α, ER-β, and PR on immunohistochemistry staining. Conclusion: Mice model at Group C has greater ER-α, ER-β, and PR expression than mice at Group A and Group B. The injection of supernatant derived from adenomyosis (Group C) to be the best model to develop mice model of endometriosis.