Effect of dayak onion tuber (Eleutherine Americana merr.) extract to prevent increased necrosis of kidney tubular epithelial cells in mice (mus musculus) to oral lead acetate exposure

Objective: This study aims to determine the effect of Dayak onion tuber extract to prevent an increase in necrosis of kidney proximal tubular epithelial cells exposed to lead acetate. Methods: Fresh Dayak onion tuber was extracted with 96% ethanol and then given orally to mice with doses of 65 mg/kg, 130 mg/kg, and 260 mg/kg for 21 days, accompanied by 14 days of lead acetate on the 8^th–21^st days. Histological preparations of the mice’s kidney were made by hematoxylin and eosin staining, and then, the necrosis of tubular cells was examined using a ×400 magnification light microscope that observed 5 views randomly marked by the presence of pyknosis, karyorrhexis, and karyolysis. Results: This study showed the higher the dose of Dayak onion tuber extract and the lower the number of tubular epithelial cell necrosis (p<0.05). Conclusion: Extract of Dayak onion tuber could prevent an increase in necrosis of kidney proximal tubular cells of mice that exposed to lead acetate.