Effect of Combination of Red Pine and Ca(OH)2 as Root Canal Sealer on Fibroblast Cell Apoptosis Through Caspase-3 Expression

Tamara Yuanita, Sylvia Paulina Panggono, Devi Eka Juniarti, Setyabudi, Dian Agustin Wahjuningrum, Iin Indah Aris Wati, Adinda Fazzahra Salma, Michele Callea, Niraj Kinariwala

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium hydroxide is one of the sealers used in root canal treatment, as it qualifies obturation, but hiroxl ions released into free radicals can damage cells. The content of flavonoids, tannins, and phenols in Red pine is a high anti-acidant to ward off free radicals. This study is expected to show the effect of a combination of red pine and Ca(OH)2 as a root canal sealer on fibroblast cells through caspase-3 expression, so that red pine can be considered as an alternative root canal sealer material. 27 wistar rats were divided into 3 groups. The control group was not treated, the kalsium hidroksidae group and the red pine group Ca(OH)2. Each group in the back area was given material and on the 3rd day an incision was made ± 1cm then a slide was made and stained with HE and IHC. There were significant differences between each group, caspase-3 expression was lower in the red pine ca(OH)2 group than the kalsium hidroksidae and control groups. The combination of Red pine and Ca(OH)2 as a root canal sealer may decrease caspase-3 expression in fibroblast cells, low Caspase-3 as an indicator of no cell apoptosis.

Original languageEnglish
Pages (from-to)1483-1488
Number of pages6
JournalJournal of International Dental and Medical Research
Volume16
Issue number4
Publication statusPublished - 2023

Keywords

  • apoptosis
  • Caspase-3
  • fibroblast cells
  • Immunology
  • Red pine
  • sealer

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