TY - JOUR
T1 - Effect of Combination of Red Pine and Ca(OH)2 as Root Canal Sealer on Fibroblast Cell Apoptosis Through Caspase-3 Expression
AU - Yuanita, Tamara
AU - Panggono, Sylvia Paulina
AU - Juniarti, Devi Eka
AU - Setyabudi,
AU - Wahjuningrum, Dian Agustin
AU - Wati, Iin Indah Aris
AU - Salma, Adinda Fazzahra
AU - Callea, Michele
AU - Kinariwala, Niraj
N1 - Publisher Copyright:
© 2023, University of Dicle. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Calcium hydroxide is one of the sealers used in root canal treatment, as it qualifies obturation, but hiroxl ions released into free radicals can damage cells. The content of flavonoids, tannins, and phenols in Red pine is a high anti-acidant to ward off free radicals. This study is expected to show the effect of a combination of red pine and Ca(OH)2 as a root canal sealer on fibroblast cells through caspase-3 expression, so that red pine can be considered as an alternative root canal sealer material. 27 wistar rats were divided into 3 groups. The control group was not treated, the kalsium hidroksidae group and the red pine group Ca(OH)2. Each group in the back area was given material and on the 3rd day an incision was made ± 1cm then a slide was made and stained with HE and IHC. There were significant differences between each group, caspase-3 expression was lower in the red pine ca(OH)2 group than the kalsium hidroksidae and control groups. The combination of Red pine and Ca(OH)2 as a root canal sealer may decrease caspase-3 expression in fibroblast cells, low Caspase-3 as an indicator of no cell apoptosis.
AB - Calcium hydroxide is one of the sealers used in root canal treatment, as it qualifies obturation, but hiroxl ions released into free radicals can damage cells. The content of flavonoids, tannins, and phenols in Red pine is a high anti-acidant to ward off free radicals. This study is expected to show the effect of a combination of red pine and Ca(OH)2 as a root canal sealer on fibroblast cells through caspase-3 expression, so that red pine can be considered as an alternative root canal sealer material. 27 wistar rats were divided into 3 groups. The control group was not treated, the kalsium hidroksidae group and the red pine group Ca(OH)2. Each group in the back area was given material and on the 3rd day an incision was made ± 1cm then a slide was made and stained with HE and IHC. There were significant differences between each group, caspase-3 expression was lower in the red pine ca(OH)2 group than the kalsium hidroksidae and control groups. The combination of Red pine and Ca(OH)2 as a root canal sealer may decrease caspase-3 expression in fibroblast cells, low Caspase-3 as an indicator of no cell apoptosis.
KW - apoptosis
KW - Caspase-3
KW - fibroblast cells
KW - Immunology
KW - Red pine
KW - sealer
UR - http://www.scopus.com/inward/record.url?scp=85182433079&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85182433079
SN - 1309-100X
VL - 16
SP - 1483
EP - 1488
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -