Effect of antibacterial activity and analysis of active compounds of Hyrtios erecta sponge ethanol extract using maceration method

This type of sponge Hyrtios erecta has not been widely studied as an antibacterial agent. The method used to extract the Hyrtios erecta sponge is using maceration. The use of this maceration method aims to compare the use of extraction in producing yields. This study used tests such as water content test using the Loss on Drying method, yield test, phytochemical test using the tube method, and antibacterial activity test using the disc diffusion method (Kirby Bauer). The research design used was RAL. Antibacterial activity data using ANOVA. While the data from the other tests were analyzed by descriptive method. The results showed that the use of different extraction methods and extract concentrations resulted in significantly different diameters of the inhibition zones. The results showed that the best treatment in producing the diameter of the inhibition zone was the maceration method with a concentration of 30000 ppm/disk which was 7.08±1.81 for Escherichia coli and 2.83±1.21 for Staphylococcus aureus. Hyrtios erecta extract was more sensitive to gram negative than gram positive. The ethanol extract of the Hyrtios erecta sponge which has been tested in the form of phytochemicals shows the presence of bioactive compounds of alkaloids, flavonoids, and saponins.