TY - JOUR
T1 - Effect of amniotic membrane-derived mesenchymal stem cells on TNF-α expression and inflammatory cells infiltration during vesicovaginal fistule repair healing process
AU - Kurniawati, Eighty Mardiyan
AU - Agung Nur Rachman, W.
AU - Santoso, Budi
AU - Parathon, Harry
AU - Widjiati,
AU - Rantam, Fedik A.
AU - Hastomo, Tri
N1 - Publisher Copyright:
© 2020, Indian Journal of Forensic Medicine and Toxicology. All rights reserved.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical and psychological condition. Until now, surgery is the mainstay treatment for this condition. However, prolonged exposure during inflammatory phase after surgery is still a problem in the healing process. TNF-α as potent pro-inflammatory cytokine plays an important role by attracting inflammatory cells to wound tissue. Amniotic membrane is the source for mesenchymal stem cells that had anti-inflammatory and imunomodulatory effect. This study aims to evaluate the effect of Amniotic Membrane-Derived Mesenchymal Stem Cells (AMMSC) on TNF-α expression and inflammatory cell infiltration during VVF repair healing process in New Zealand White (NZW) rabbit model. Method: This study was an experimental study with randomized posttest only control group design. Twentyseven NZW rabbit as VVF model was used in this study, randomly divided into 3 different treatment groups after underwent surgical treatment (no treatment , treated with freeze-dried amniotic membrane , and treated with freeze-dried amniotic membrane that seeded with AMMSC ). Evaluation was done 7 days after treatment. TNF-α expression was evaluated semiquantitatively using modified Remmele-Stegner scale. Inflammatory cell infiltration was evaluated using modified Klopfleisch method. Results: Mean TNF-α expression between C, T1, and T2 group were significantly different (8.5 ± 1.6; 7.1 ± 1.2; 1.6 ± 1.2 respectively, p < 0.001). Median inflammatory cell infiltration between C, T1, and T2 group were significantly different (3.0; 2.0; 1.0 respectively, p < 0.001). Conclusion: AMMSC significantly reduced TNF-α expression and inflammatory cells infiltration during VVF repair healing process.
AB - Background: Vesicovaginal fistula (VVF) causes high morbidity in women, affecting both physical and psychological condition. Until now, surgery is the mainstay treatment for this condition. However, prolonged exposure during inflammatory phase after surgery is still a problem in the healing process. TNF-α as potent pro-inflammatory cytokine plays an important role by attracting inflammatory cells to wound tissue. Amniotic membrane is the source for mesenchymal stem cells that had anti-inflammatory and imunomodulatory effect. This study aims to evaluate the effect of Amniotic Membrane-Derived Mesenchymal Stem Cells (AMMSC) on TNF-α expression and inflammatory cell infiltration during VVF repair healing process in New Zealand White (NZW) rabbit model. Method: This study was an experimental study with randomized posttest only control group design. Twentyseven NZW rabbit as VVF model was used in this study, randomly divided into 3 different treatment groups after underwent surgical treatment (no treatment , treated with freeze-dried amniotic membrane , and treated with freeze-dried amniotic membrane that seeded with AMMSC ). Evaluation was done 7 days after treatment. TNF-α expression was evaluated semiquantitatively using modified Remmele-Stegner scale. Inflammatory cell infiltration was evaluated using modified Klopfleisch method. Results: Mean TNF-α expression between C, T1, and T2 group were significantly different (8.5 ± 1.6; 7.1 ± 1.2; 1.6 ± 1.2 respectively, p < 0.001). Median inflammatory cell infiltration between C, T1, and T2 group were significantly different (3.0; 2.0; 1.0 respectively, p < 0.001). Conclusion: AMMSC significantly reduced TNF-α expression and inflammatory cells infiltration during VVF repair healing process.
KW - Amniotic membrane mesenchymal stem cells
KW - Macrophageneutrophil
KW - TNF-α
KW - Vesicovaginal fistule
UR - http://www.scopus.com/inward/record.url?scp=85087421926&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85087421926
SN - 0973-9122
VL - 14
SP - 1518
EP - 1522
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
IS - 2
ER -