Effect of α-mangostin and Lipopolysaccharides Exposure on Osteocalcin and Interleukin-17A Expression

The early inflammatory phase, which is impacted by both local and systemic reactions to noxious stimuli, is crucial for bone regeneration. One component of Garcinia mangostana, called α-mangostin, offers several advantages, including acting as an anti-inflammatory. With the production of related transcription factor-2 (RUNX2), which in turn causes the appearance of various osteogenesis markers, the WNT signaling pathway is a mechanism for the formation of osteogenesis through inflammation. To analyze the effect of α-mangostin on the LPS-induced inflammatory process on osteocalcin and interleukin-17A gene expression in osteoblast cell line culture. This study examines the expression of the genes for IL-17A and OCN in osteoblast cell culture using a post-test-only control group design. A total of 4 groups of cell cultures were created: group K with osteogenic medium, group P1 with osteogenic medium and lipopolysaccharide, group P2 with osteogenic medium and α-mangostin, and group P3 with osteogenic medium and a combination of lipopolysaccharide and α-mangostin. Using the same concentration for all concentrates, the therapy was administered over three days in osteogenic media. The sample will subsequently be prepared and subjected to RT-PCR analysis. Result is significant upregulation of IL-17A and OCN gene expression was observed in the group combination of lipopolysaccharide and α-mangostin. In conclusion α-mangostin can boost osteocalcin gene expression in osteoblast cell line culture after LPS induction, however, it was unable to diminish IL-17A gene expression in osteoblast cell line culture.