Objectives: Osteoporosis is a condition characterized by skeletal degradation of osseous tissue resulting in an escalated chance of broken bones. Traditionally, Rusa unicolor horn from East Kalimantan is used to treat many diseases, including conditions associated with bone turnover. The aim of the present research was to analyze the effects of 70% ethanol and aqueous extracts of R. unicolor antler’s horn from East Kalimantan on nitric oxide inhibition, osteoblast differentiation, and mineralization related to bone turnover. Materials and Methods: Nitric oxide inhibition of the extracts in lipopolysaccharide-stimulated RAW 264.7 macrophages was evaluated by Griess reagent, while the effects of extracts on osteoblast differentiations were measured by alkaline phosphatase in p-nitrophenyl phosphate. Their effects on mineralization was determined using alizarin red staining. Results: The 70% ethanol and aqueous extracts inhibited cell inflammation (40% and 80%, respectively) and stimulated osteoblast differentiation (65% and 52%, respectively). In the mineralization test, the aqueous extract showed an effect two times higher than that of 70% ethanol extract. Conclusion: The extracts can effectively degrade inflammatory marker expression and preserve osteoblast functions.