TY - JOUR
T1 - Dna barcoding of invasive freshwater fish reveals two species of amphilophus from two dams in brantas stream, east java, indonesia
AU - Amin, Muhammad Hilman Fu’Adil
AU - Hayati, Alfiah
AU - Darmanto, Win
AU - Pramudya, Manikya
N1 - Publisher Copyright:
© EM International.
PY - 2019
Y1 - 2019
N2 - DNA barcode is a new DNA based identification method using cytochrome c oxidase I (COI) fragment as standard for identifying species in animal kingdom. In this study, we applied DNA barcode to identify invasive species in genus Amphilophus (Mayan Cichlid) from two dams in Brantas watershed, East Java, Indonesia. 16 samples of Amphilophus were collected from Karangkates Dam and Lahor Dam with various body color. Total genome was extracted from muscle tissue, and amplified using universal primer for COI, and sequenced. COI fragments were identified using BOLD Identification System and unrooted Neighbor-Joining (NJ) tree, pairwise Kimura 2-parameter (K2P) distance and haplotype network were performed. COI sequence analysis from this study showed that there are two species in both dams, A. citrinellus and A. trimaculatus. Pairwise distances between two species was 4.8% and number of segregating sites among samples are 31 nucleotides, demonstrating that the population consist of two species. Haplotype-body color mapping showed that A. citrinellus has more color polymorphism, from dark to bright color, compared to A. trimaculatus which have dark color. From this finding, DNA barcode is a significant method for the species identification and produce a functional framework for exploring species invasion.
AB - DNA barcode is a new DNA based identification method using cytochrome c oxidase I (COI) fragment as standard for identifying species in animal kingdom. In this study, we applied DNA barcode to identify invasive species in genus Amphilophus (Mayan Cichlid) from two dams in Brantas watershed, East Java, Indonesia. 16 samples of Amphilophus were collected from Karangkates Dam and Lahor Dam with various body color. Total genome was extracted from muscle tissue, and amplified using universal primer for COI, and sequenced. COI fragments were identified using BOLD Identification System and unrooted Neighbor-Joining (NJ) tree, pairwise Kimura 2-parameter (K2P) distance and haplotype network were performed. COI sequence analysis from this study showed that there are two species in both dams, A. citrinellus and A. trimaculatus. Pairwise distances between two species was 4.8% and number of segregating sites among samples are 31 nucleotides, demonstrating that the population consist of two species. Haplotype-body color mapping showed that A. citrinellus has more color polymorphism, from dark to bright color, compared to A. trimaculatus which have dark color. From this finding, DNA barcode is a significant method for the species identification and produce a functional framework for exploring species invasion.
KW - COI
KW - DNA Barcoding
KW - Mayan cichlids
UR - http://www.scopus.com/inward/record.url?scp=85071765247&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85071765247
SN - 0971-765X
VL - 25
SP - S141-S145
JO - Ecology, Environment and Conservation
JF - Ecology, Environment and Conservation
IS - 7
ER -