TY - JOUR
T1 - Distribution of mycobacterium lepraegenotypes from surabaya and bandung clinical isolates by multiple locus variable number of tandem repeat analysis
AU - Prakoeswa, Cita Rosita Sigit
AU - Rumondor, Bayu Bijaksana
AU - Damayanti, Lina
AU - Sasmojo, Muljaningsih
AU - Adriaty, Dinar
AU - Alinda, Medhi Denisa
AU - Wahyuni, Ratna
AU - Listiawan, M. Yulianto
AU - Agusni, Indropo
AU - Izumi, Shinzo
N1 - Publisher Copyright:
© Copyright C.R.S. Prakoeswa, et al., 2019.
PY - 2019
Y1 - 2019
N2 - Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis has been proposed as a means of genotyping for tracking leprosy transmission due to tandem repeats' potential as genetic markers to differentiate M. leprae strains. However, characteristics of polymorphism can vary depending on the population. This study aimed to compare the copy number of repeats in four genetic markers: TTC, AC8a, AC9 and 6-7 in leprosy patients from Surabaya and Bandung. Twenty three patients from Dr. Soetomo General Hospital and 21 from Hasan Sadikin Hospital were recruited. Multiple locus VNTR analysis was applied using total DNA extracts from Slit Skin Smear (SSS). From Surabaya, 7 samples showed the same copy number of four genetic markers (TTC=15; AC8a=10; AC9=10 and 6-7=6) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6); as for samples from Bandung, 2 showed the same copy number (TTC=15; AC8a=8; AC9=10 and 6-7=8) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6). The multiple locus VNTR analysis showed two identical M. leprae VNTR profiles from Bandung and Surabaya which supports the use of VNTR loci for transmission studies.
AB - Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis has been proposed as a means of genotyping for tracking leprosy transmission due to tandem repeats' potential as genetic markers to differentiate M. leprae strains. However, characteristics of polymorphism can vary depending on the population. This study aimed to compare the copy number of repeats in four genetic markers: TTC, AC8a, AC9 and 6-7 in leprosy patients from Surabaya and Bandung. Twenty three patients from Dr. Soetomo General Hospital and 21 from Hasan Sadikin Hospital were recruited. Multiple locus VNTR analysis was applied using total DNA extracts from Slit Skin Smear (SSS). From Surabaya, 7 samples showed the same copy number of four genetic markers (TTC=15; AC8a=10; AC9=10 and 6-7=6) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6); as for samples from Bandung, 2 showed the same copy number (TTC=15; AC8a=8; AC9=10 and 6-7=8) and 2 showed another (TTC=16; AC8a=10; AC9=11 and 6-7=6). The multiple locus VNTR analysis showed two identical M. leprae VNTR profiles from Bandung and Surabaya which supports the use of VNTR loci for transmission studies.
KW - Bandung
KW - Genotyping
KW - M. leprae
KW - Slit skin smear
KW - Surabaya
KW - Variable number of tandem repeat
UR - http://www.scopus.com/inward/record.url?scp=85071450697&partnerID=8YFLogxK
U2 - 10.4081/dr.2019.8017
DO - 10.4081/dr.2019.8017
M3 - Article
AN - SCOPUS:85071450697
SN - 2036-7392
VL - 11
SP - 14
EP - 18
JO - Dermatology Reports
JF - Dermatology Reports
IS - S1
ER -