TY - JOUR
T1 - Discrimination of Curcuma species from Asia using intron length polymorphism markers in genes encoding diketide-CoA synthase and curcumin synthase
AU - Liu, Qundong
AU - Zhu, Shu
AU - Hayashi, Shigeki
AU - Iida, Osamu
AU - Takano, Akihito
AU - Miyake, Katsunori
AU - Sukrong, Suchada
AU - Agil, Mangestuti
AU - Balachandran, Indira
AU - Nakamura, Norio
AU - Kawahara, Nobuo
AU - Komatsu, Katsuko
N1 - Publisher Copyright:
© 2021, The Japanese Society of Pharmacognosy.
PY - 2022/1
Y1 - 2022/1
N2 - Recently, Curcuma rhizome-related foods with claimed health benefits have been used worldwide; however, correct identification and quality assessment have not been conducted. Due to the wide distribution and morphological similarities of Curcuma species, the classification of some species is debated and nomenclature is inconsistent among countries. In this study, to elucidate specific molecular markers of medicinally used Curcuma species in Asia, and to solve the confusion on the reported botanical origin of crude drugs, molecular analysis based on the intron length polymorphism (ILP) in genes encoding diketide-CoA synthase and curcumin synthase and the trnK intron sequences was performed using 59 plant specimens and 42 crude drug samples from 13 Curcuma species, obtained from Asian countries. The ILP patterns of the respective species from both plant specimens and crude drug samples revealed high consistency in C. aromatica, C. zedoaria, C. phaeocaulis, C. aeruginosa, C. wenyujin, and C. zanthorrhiza, but showed intraspecies polymorphism in C. longa, C. kwangsiensis, C. amada, C. mangga and C. comosa. The C. longa specimens and samples were separated into three subgroups which were highly consistent with their geographical origins. Based on the ILP markers and the trnK intron sequences, the botanical origins of “Khamin oi” from Thailand were correctly determined to be C. longa or a hybrid between C. longa and other species, and “Wan narn kum” from Thailand and “Kasturi manjal” from India were correctly determined to be C. zanthorrhiza.
AB - Recently, Curcuma rhizome-related foods with claimed health benefits have been used worldwide; however, correct identification and quality assessment have not been conducted. Due to the wide distribution and morphological similarities of Curcuma species, the classification of some species is debated and nomenclature is inconsistent among countries. In this study, to elucidate specific molecular markers of medicinally used Curcuma species in Asia, and to solve the confusion on the reported botanical origin of crude drugs, molecular analysis based on the intron length polymorphism (ILP) in genes encoding diketide-CoA synthase and curcumin synthase and the trnK intron sequences was performed using 59 plant specimens and 42 crude drug samples from 13 Curcuma species, obtained from Asian countries. The ILP patterns of the respective species from both plant specimens and crude drug samples revealed high consistency in C. aromatica, C. zedoaria, C. phaeocaulis, C. aeruginosa, C. wenyujin, and C. zanthorrhiza, but showed intraspecies polymorphism in C. longa, C. kwangsiensis, C. amada, C. mangga and C. comosa. The C. longa specimens and samples were separated into three subgroups which were highly consistent with their geographical origins. Based on the ILP markers and the trnK intron sequences, the botanical origins of “Khamin oi” from Thailand were correctly determined to be C. longa or a hybrid between C. longa and other species, and “Wan narn kum” from Thailand and “Kasturi manjal” from India were correctly determined to be C. zanthorrhiza.
KW - Curcuma
KW - Curcumin synthase
KW - Diketide-CoA synthase
KW - Intron length polymorphism
KW - Molecular identification
KW - trnK
UR - http://www.scopus.com/inward/record.url?scp=85114358202&partnerID=8YFLogxK
U2 - 10.1007/s11418-021-01558-2
DO - 10.1007/s11418-021-01558-2
M3 - Article
AN - SCOPUS:85114358202
SN - 1340-3443
VL - 76
SP - 69
EP - 86
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 1
ER -