TY - GEN
T1 - Direct conversion of starch to ethanol using recomblnant Saccharomyces cerevisiae containing glucoamylase gene
AU - Purkan, P.
AU - Baktir, A.
AU - Puspaningsih, N. N.T.
AU - Ni'Mah, M.
N1 - Funding Information:
The research was supported by Department of Natıonal Educatıon, Dırectorate General of Hıgher Educatıon, Southeast Asıan Regıonal Centre For Tropıcal Bıology, Finantial year 2008. We would also thank to National BioResources Project (NBRP) Yeast, which suppported S. Cerevisiae BY5207 yeast; and Cemistry Departmenet, Faculty of Mathematic and Natural Sciences. Institute Technology Bandung which suppported S. cerevisiae AS3324 yeast.
Publisher Copyright:
© 2017 Author(s).
PY - 2017/9/21
Y1 - 2017/9/21
N2 - Saccharomyces cerevisiae is known for its high fermentative capacity, high ethanol yield and its high ethanol tolerance. The yeast is inability converting starch (relatively inexpensive substrate) into biofuel ethanol. Insertion of glucoamylase gene in yeast cell of Saccharomyces cerevisiae had been done to increase the yeast function in ethanol fermentation from starch. Transformation of yeast of S. cerevisiae with recombinant plasmid yEP-GLO1 carrying gene encoding glucoamylase (GLO1) produced the recombinant yeast which enable to degrade starch. Optimizing of bioconversion process of starch into ethanol by the yeast of recombinant Saccharomyces cerevisiae [yEP-GLO1] had been also done. Starch concentration which could be digested by recombinant yeast of S. cerevisiae [yEP-GLO1] was 10% (w/v). Bioconversion of starch having concentration 10% (b/v) using recombinant yeast of S. cerevisiae BY5207 [yEP-GLO1] could result ethanol as 20% (v/v) to alcoholmeter and 19,5% (v/v) to gas of chromatography. Otherwise, using recombinant yeast S. cerevisiae S. cerevisiae AS3324 [yEP-GLO1] resulted ethanol as 17% (v/v) to alcoholmeter and 17,5% (v/v) to gas of chromatography. The highest ethanol in starch bioconversion using both recombinant yeasts BY5207 and AS3324 could be resulted on 144 hours of fermentation time as well as in pH 5.
AB - Saccharomyces cerevisiae is known for its high fermentative capacity, high ethanol yield and its high ethanol tolerance. The yeast is inability converting starch (relatively inexpensive substrate) into biofuel ethanol. Insertion of glucoamylase gene in yeast cell of Saccharomyces cerevisiae had been done to increase the yeast function in ethanol fermentation from starch. Transformation of yeast of S. cerevisiae with recombinant plasmid yEP-GLO1 carrying gene encoding glucoamylase (GLO1) produced the recombinant yeast which enable to degrade starch. Optimizing of bioconversion process of starch into ethanol by the yeast of recombinant Saccharomyces cerevisiae [yEP-GLO1] had been also done. Starch concentration which could be digested by recombinant yeast of S. cerevisiae [yEP-GLO1] was 10% (w/v). Bioconversion of starch having concentration 10% (b/v) using recombinant yeast of S. cerevisiae BY5207 [yEP-GLO1] could result ethanol as 20% (v/v) to alcoholmeter and 19,5% (v/v) to gas of chromatography. Otherwise, using recombinant yeast S. cerevisiae S. cerevisiae AS3324 [yEP-GLO1] resulted ethanol as 17% (v/v) to alcoholmeter and 17,5% (v/v) to gas of chromatography. The highest ethanol in starch bioconversion using both recombinant yeasts BY5207 and AS3324 could be resulted on 144 hours of fermentation time as well as in pH 5.
UR - http://www.scopus.com/inward/record.url?scp=85030786936&partnerID=8YFLogxK
U2 - 10.1063/1.5004318
DO - 10.1063/1.5004318
M3 - Conference contribution
AN - SCOPUS:85030786936
T3 - AIP Conference Proceedings
BT - 6th International Conference and Workshops on Basic and Applied Sciences
A2 - Abdullah, Hewa Y.
PB - American Institute of Physics Inc.
T2 - 6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017
Y2 - 18 March 2017 through 19 March 2017
ER -