Direct conversion of starch to ethanol using recomblnant Saccharomyces cerevisiae containing glucoamylase gene

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Abstract

Saccharomyces cerevisiae is known for its high fermentative capacity, high ethanol yield and its high ethanol tolerance. The yeast is inability converting starch (relatively inexpensive substrate) into biofuel ethanol. Insertion of glucoamylase gene in yeast cell of Saccharomyces cerevisiae had been done to increase the yeast function in ethanol fermentation from starch. Transformation of yeast of S. cerevisiae with recombinant plasmid yEP-GLO1 carrying gene encoding glucoamylase (GLO1) produced the recombinant yeast which enable to degrade starch. Optimizing of bioconversion process of starch into ethanol by the yeast of recombinant Saccharomyces cerevisiae [yEP-GLO1] had been also done. Starch concentration which could be digested by recombinant yeast of S. cerevisiae [yEP-GLO1] was 10% (w/v). Bioconversion of starch having concentration 10% (b/v) using recombinant yeast of S. cerevisiae BY5207 [yEP-GLO1] could result ethanol as 20% (v/v) to alcoholmeter and 19,5% (v/v) to gas of chromatography. Otherwise, using recombinant yeast S. cerevisiae S. cerevisiae AS3324 [yEP-GLO1] resulted ethanol as 17% (v/v) to alcoholmeter and 17,5% (v/v) to gas of chromatography. The highest ethanol in starch bioconversion using both recombinant yeasts BY5207 and AS3324 could be resulted on 144 hours of fermentation time as well as in pH 5.

Original languageEnglish
Title of host publication6th International Conference and Workshops on Basic and Applied Sciences
EditorsHewa Y. Abdullah
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415713
DOIs
Publication statusPublished - 21 Sep 2017
Event6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017 - Erbil, Kurdistan, Iraq
Duration: 18 Mar 201719 Mar 2017

Publication series

NameAIP Conference Proceedings
Volume1888
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference6th International Conference and Workshops on Basic and Applied Sciences, ICOWOBAS 2017
Country/TerritoryIraq
CityErbil, Kurdistan
Period18/03/1719/03/17

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