@article{c0d2916f441e4a9da2cc84ba648c7f47,
title = "Differential effects of chromosome and plasmid blaCTX-M-15 genes on antibiotic susceptibilities in extended-spectrum beta-lactamase-producing Escherichia coli isolates from patients with urinary tract infection",
abstract = "Objectives: To compare antibiotic susceptibilities between chromosomal and plasmid blaCTX-M-15 locations in urinary tract infection-causing extended-spectrum β-lactamases-producing Escherichia coli blaCTX-M-15 isolated in Indonesia. Methods: A total of 84 strains identified as extended-spectrum β-lactamases-producing E. coli were isolated from patients with urinary tract infection in Indonesia in 2015. Antimicrobial susceptibility tests were performed on these strains using 18 antibiotics, and extended-spectrum β-lactamase bla genes were detected by polymerase chain reaction. Gene localization of blaCTX-M-15-positive strains was confirmed by Southern blot hybridization, and epidemiological typing was conducted using multilocus sequence typing. Results: Of 54 strains harboring the blaCTX-M-15 gene, 27 showed localization on chromosome, 20 on plasmid, and seven on chromosome and plasmid. Most multilocus sequence typing sequence types of the 27 strains with chromosomal blaCTX-M-15 were ST405 (25.9%) and ST131 (22.2%) strains, whereas the 20 strains with plasmid-blaCTX-M-15 were mostly ST410 (55.0%). Conclusions: Extended-spectrum β-lactamases-producing E. coli blaCTX-M-15 with plasmid genes show significantly higher resistant rates against piperacillin-tazobactam but lower resistant rates against chloramphenicol compared to chromosomal strains in Indonesian patients with urinary tract infection. Mechanistic investigations will be necessary to advance our knowledge of antimicrobial resistance in urinary tract infection.",
keywords = "CTX-M-15, Escherichia coli, chromosomal localization, extended-spectrum β-lactamases (ESBL)",
author = "Yang, {Young min} and Kayo Osawa and Koichi Kitagawa and Samiko Hosoya and Reo Onishi and Aya Ishii and Toshiro Shirakawa and Itaru Hirai and Kuntaman Kuntaman and Hiroshi Tanimoto and Katsumi Shigemura and Masato Fujisawa",
note = "Funding Information: This study was supported by grants from the Japan Initiative for Global Research Network on Infectious Diseases (J‐GRID), the Ministry of Education, Culture, Sport, Science and Technology in Japan (Grant No. 18fm0108004h0004), the Japan Agency for Medical Research and Development (AMED; Grant No. JP18fk0108019]), the JSPS KAKENHI (Grant No. 18K10045 and 19K09670), the Tahir Professorship from the University of Airlangga, Surabaya, Indonesia, the Institute of Tropical Disease, University of Airlangga, Surabaya, Indonesia, and the Indonesia‐Japan Collaborative Research Center for Emerging and Re‐emerging Infectious Diseases. We would like to thank the following for collaborations: Shohiro Kinoshita (Division of Biophysics, Kobe University Graduate School of Health Sciences); Ni Made Mertaniasih (Department of Microbiology, Faculty of Medicine, Airlangga University); Usman Hadi (Department of Microbiology, Faculty of Medicine, Airlangga University); and Dadik Rahadjo (Institute of Tropical Disease, Airlangga University, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University). Funding Information: This study was supported by grants from the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), the Ministry of Education, Culture, Sport, Science and Technology in Japan (Grant No. 18fm0108004h0004), the Japan Agency for Medical Research and Development (AMED; Grant No. JP18fk0108019]), the JSPS KAKENHI (Grant No. 18K10045 and 19K09670), the Tahir Professorship from the University of Airlangga, Surabaya, Indonesia, the Institute of Tropical Disease, University of Airlangga, Surabaya, Indonesia, and the Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases. We would like to thank the following for collaborations: Shohiro Kinoshita (Division of Biophysics, Kobe University Graduate School of Health Sciences); Ni Made Mertaniasih (Department of Microbiology, Faculty of Medicine, Airlangga University); Usman Hadi (Department of Microbiology, Faculty of Medicine, Airlangga University); and Dadik Rahadjo (Institute of Tropical Disease, Airlangga University, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University). Publisher Copyright: {\textcopyright} 2021 The Japanese Urological Association",
year = "2021",
month = jun,
doi = "10.1111/iju.14498",
language = "English",
volume = "28",
pages = "623--628",
journal = "International Journal of Urology",
issn = "0919-8172",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "6",
}