TY - JOUR
T1 - Diagnostic value of helicobacter pylori serum serology using immunochromatography method with current infection marker compared to histopathology in dyspeptic patients
AU - Miftahussurur, Muhammad
AU - Syalini, Denada Aisyah
AU - Nusi, Iswan Abbas
AU - Maimunah, Ummi
AU - Kahar, Hartono
AU - Setiawan, Poernomo Boedi
AU - Purbayu, Herry
AU - Kholili, Ulfa
AU - Widodo, Budi
AU - Thamrin, Husin
AU - Vidyani, Amie
AU - Siregar, Gontar Alamsyah
AU - Rezkitha, Yudith Annisa Ayu
AU - Sugihartono, Titong
N1 - Funding Information:
This study was funded by grants from the Riset Mandat 2019 Grants from Universitas Airlangga (340/UN.3.14/LT/2019).
Publisher Copyright:
© 2020 Miftahussurur et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Introduction: Examination using Outer Membrane Protein as a marker of active Helicobacter pylori Current Infection Marker (CIM) infection has now been developed. However, the accuracy of CIM is still unknown. This study aimed to analyze the diagnostic value of serum serology using CIM compared to histopathological examination as the gold standard for diagnosing the presence of H. pylori infection in dyspepsia patients. Methods: This study involved fifty-two subjects with dyspepsia. Endoscopic, biopsy, and histopathological examination with modified-giemsa staining as gold standard and serological examination using immunochromatography method with CIM (AssureR, Singapore) had been done to all subjects of this study. Results: Pre-test probability of H. pylori infection was 17.3%. Most of subjects infected with H. pylori were male with average age of 53.89±7.75 years old. Most of endoscopic features of subjects infected with H. pylori were erosive gastritis. The sensitivity, specificity, positive predictive value, negative predictive value of CIM were 22.2%, 95.3%, 50%, and 85.4%, respectively. The positive likelihood ratio, negative likelihood ratio, and accuracy of CIM were 4.8, 0.8, and 82.7%, respectively. Conclusion: Serum serology using immunochromatography with CIM cannot replace histopathology for diagnosis of Helicobacter pylori current infection in dyspeptic patients.
AB - Introduction: Examination using Outer Membrane Protein as a marker of active Helicobacter pylori Current Infection Marker (CIM) infection has now been developed. However, the accuracy of CIM is still unknown. This study aimed to analyze the diagnostic value of serum serology using CIM compared to histopathological examination as the gold standard for diagnosing the presence of H. pylori infection in dyspepsia patients. Methods: This study involved fifty-two subjects with dyspepsia. Endoscopic, biopsy, and histopathological examination with modified-giemsa staining as gold standard and serological examination using immunochromatography method with CIM (AssureR, Singapore) had been done to all subjects of this study. Results: Pre-test probability of H. pylori infection was 17.3%. Most of subjects infected with H. pylori were male with average age of 53.89±7.75 years old. Most of endoscopic features of subjects infected with H. pylori were erosive gastritis. The sensitivity, specificity, positive predictive value, negative predictive value of CIM were 22.2%, 95.3%, 50%, and 85.4%, respectively. The positive likelihood ratio, negative likelihood ratio, and accuracy of CIM were 4.8, 0.8, and 82.7%, respectively. Conclusion: Serum serology using immunochromatography with CIM cannot replace histopathology for diagnosis of Helicobacter pylori current infection in dyspeptic patients.
KW - Current infection marker
KW - Dyspepsia
KW - Helicobacter pylori
KW - Immunochromatography
UR - http://www.scopus.com/inward/record.url?scp=85090647604&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85090647604
SN - 1307-9867
VL - 14
SP - 1869
EP - 1876
JO - EurAsian Journal of BioSciences
JF - EurAsian Journal of BioSciences
IS - 1
ER -