Diagnosis of single and twin pregnancy, and early embryo mortality through progesterone level test on local doEs

The purpose of the study was to determine the levels of progesterone to diagnose single and twin pregnancy, early embryonic mortality and in improving livestock productivity. Twenty does were estrous synchronized using PGF2α twice and inseminated using fresh semen of etawa buck, and blood sampling for progesterone levels were examined with Radio immunoassay (RIA). The results of the progesterone before pregnancy, at days 14 and 21 after AI in single pregnancy, twins, triplets and quadruplets were 0.84±1.47 ng/ml up to 1.56±1.27 ng/ml, 2.5+ 0.16 ng/ml up to 2.91+0.51 ng/ml, 2.64+0.56 ng/ml up to 3.61 + 0.13 ng/ml, 2.89±0.83 ng/ml up to 4.05±1.23 ng/ ml abd 3.08±0.72 ng/ml up to 4.39±1.21 ng/ml respectively. Differences in progesterone levels can be used to determine early mortality of embryos and late implantation occurs in single kid and twins <1 ng/ml at 6^th days.