Development of nonalcoholic fatty liver disease model by high-fat diet in rats

Hijrawati Ayu Wardani, Mahardian Rahmadi, Chrismawan Ardianto, Santhra Segaran Balan, Norshafarina Shari Kamaruddin, Junaidi Khotib

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17 Citations (Scopus)


Nonalcoholic fatty liver disease (NAFLD) is indicated by liver steatosis without excessive alcohol use or other liver disease. Several studies have reported that metabolic syndromes such as obesity, type 2 diabetes mellitus, and dyslipidemia have a linear correlation associated with NAFLD pathophysiology. One of the characteristics of dyslipidemia in NAFLD is increase in serum triglycerides. This study aimed to develop a model of NAFLD characterized by an increase in serum triglyceride levels and histological profile of liver steatosis by high-fat diet in rats. Twelve Wistar rats were fed with pellets enriched with 60% fat. They were housed individually, and the remaining pellets were weighted every day for intake evaluation. Blood samples were collected at day 0 and at the end of each trial period at days 7, 14, 21, and 28 for the measurement of triglyceride levels. Every animal from each group was also sacrificed for liver histopathological examination. This study has established developing the NAFLD animal model by induction of a high-fat diet. The levels of serum triglycerides were increased from baseline 80.41 ± 12.82 to 1152.00 ± 73.62, 493.66 ± 159.98, 556.00 ± 120.79, and 489.00 ± 156.75 mg/dL at days 7, 14, 21, and 28, respectively. Liver histology also showed liver steatosis development, inflammation, and hepatocellular ballooning, which were associated with the NAFLD state. High-fat diet in rats induced hypertriglyceridemia along with NAFLD-like liver histopathology.

Original languageEnglish
Article number20190258
JournalJournal of Basic and Clinical Physiology and Pharmacology
Issue number6
Publication statusPublished - 1 Nov 2020


  • high-fat diet
  • hypertriglyceridemia
  • nonalcoholic fatty liver disease


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